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[求助]
关于荧光传感薄膜的几个问题
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现在我正在做关于荧光传感薄膜，遇到了几个问题，想请高人指点： 1. 在测膜的荧光的时候，是否要重点考虑膜表面的漫反射情况？ 2. 在第一个问题的基础上，几张膜经过不同的处理之后，测得的荧光强度来荧光强度是否可以比较？ 3. 如果荧光分子是靠静电作用发生的荧光淬灭，那么，该过程的速度快吗？ 谢谢！ |
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sally208
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(1) If fluorophores are embedded inside the film, you should consider the possbile loss of light owing the scattering/reflection/absorption of film materials. (2) It depends on how you treat with these films. If the optical properties do not change significantly, I think you can make a rough comparison. (3) It depends on how fast these quenchers can diffuse into the film for interaction. The charge, size of 'quenchers' can all affect the result.  |
2楼2012-03-15 21:45:06
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Thank you！ In some studies, fluorophores are indeed embedded inside the film whose thickness is ca. 10 μm, they used this film as sensors to detect pH via measuring the change of fluorescence intensity. And during the process, they fix position of film making sure that lase rpulse irradiates at the same position. My question is "whether this means can be used to accurately detect pH as a sendor or not". |
3楼2012-03-19 09:32:23
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