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PhD position
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Protein folding and maturation can differ substantially between newly synthesized proteins and in vitro refolded proteins. In classical folding studies, formerly folded proteins need to be unfolded before the (re-)folding process can be studied. However it has been shown that protein folding takes place already during the elongation of the nascent chain (co-translational folding). Therefore, one major goal is to understand how polypeptide chain elongation and folding are coupled. Single-molecule studies can yield valuable information about these processes. In this respect we are looking for a Ph.D. student to join our research on single molecule protein folding. In this project the candidate will have the chance to conduct interdisciplinary research with focus in molecular biology and fluorescence microscopy. He/she will design constructs and study them in the fluorescence microscope in order to get a better insight of cotranslational protein folding in a single molecule level. The work will be conducted in the Forschungszentrum Jülich, one of the largest and best equipped research centers in Europe, which offers a highly interdisciplinary environment. Our group is taking part in the International Helmholtz Research School of Biophysics and Soft Matter (IHRS BioSoft) and a suitable candidate has also the chance to conduct his Ph.D. through this research school. Methoden: Molecular biology and biochemical methods Fluorescence microscopy Anfangsdatum: 1. Juni 2012 geschätzte Dauer: 3 years Bezahlung: TVöD 13 65% Veröffentlichungen: A. Katranidis, D. Atta, R. Schlesinger, K.H. Nierhaus, T. Choli-Papadopoulou, I. Gregor, M. Gerrits, G. Büldt and J. Fitter. (2009) Fast biosynthesis of GFP molecules - a single molecule fluorescence study. Angewandte Chemie Int. Edit. 48, 1758-1761 Katranidis, A., Grange, W., Schlesinger, R., Choli-Papadopoulou, T., Brüggemann, D., Hegner, M. and Büldt, G. (2011). Force Measurements of the Disruption of the Nascent Polypeptide Chain from the Ribosome by Optical Tweezers FEBS Letters 585, 1859-1863 Homepage: http://www.fz-juelich.de/ics/ics ... ikel.html?nn=762230 The ideal candidate should have a Diploma or Master degree in Biology, Chemistry or Natural Sciences with a strong background in Molecular Biology and Biochemistry. Basic knowledge of fluorescence microscopy is advantageous. Good communicational skills in English are a requirement. The applicant should be highly motivated, should have an enthusiastic can-do attitude and an enquiring and creative mind. Contact Prof. Dr. Fitter Email: j.fitter@fz-juelich.de Tel: 02461 612024 Dr. Katranidis Email: a.katranidis@fz-juelich.de Tel: 02461 612036 |
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