| 查看: 544 | 回复: 3 | ||
| 当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖 | ||
[求助]
求助一段英语翻译。。。急啊。。。
|
||
| Amicetin, an antibacterial and antiviral agent, belongs to a group of disaccharide nucleoside antibiotics featuring an α -(1→4)-glycoside bond in the disaccharide moiety. In this study, the amicetin biosynthetic gene cluster was cloned from Streptomyces vinaceus-drappus NRRL 2363 and localized on a 37 kb contiguous DNA region. Heterologous expression of the amicetin biosynthetic gene cluster in Streptomyces livdians TK64 resulted in the production of amicetin and its analogues, thereby confirming the identity of the ami -gene cluster. In silico sequence analysis revealed that 21 genes were putatively involved in amicetin biosynthesis, including 3 for regulation and transportation, 10 for disaccharide biosynthesis, and 8 for the formation of amicetin skeleton by the linkage of cytosine, para-benzoic acid (PABA), and the terminal (+)-α -methylserine moieties. The inactivation of the benzoate-CoA ligase gene amiL and the N-acetyltransferase gene amiF, led to two mutants, which accumulated the same two compounds cytosamine and 4-acetamido-3- hydroxybenzoic acid. These data indicated that AmiF functioned as an amide synthethase to link cytosine and PABA. The inactivation of amiR, encoding an acyl CoA-acyl carrier protein transacylase, resulted in the production of plicacetin and norplicacetin, indicating AmiR to be responsible for attachment of the terminal methylserine moiety to form another amide bond. These findings implicated two alternative strategies for amide bond formation in amicetin biosynthesis. |
回复此楼» 猜你喜欢
一志愿085404，总分291，四级已过，求调剂
已经有9人回复
-
285求调剂
已经有6人回复
-
266求调剂
已经有8人回复
-
材料专硕调剂
已经有9人回复
-
化学工程调剂289
已经有21人回复
-
机械调剂
已经有3人回复
-
368化学求调剂
已经有4人回复
-
0703调剂，一志愿天津大学319分
已经有21人回复
-
0854电子信息319求调剂（接受跨专业调剂）
已经有6人回复
-
调剂
已经有15人回复
4楼2012-02-01 12:42:19
2楼2012-01-31 14:06:20
zuodg
铁杆木虫 (正式写手)
有为青年
- 翻译EPI: 180
- 应助: 5 (幼儿园)
- 贵宾: 0.015
- 金币: 6765.2
- 散金: 48
- 红花: 18
- 帖子: 500
- 在线: 285.5小时
- 虫号: 1055187
- 注册: 2010-07-10
- 性别: GG
- 专业: 外国语言
3楼2012-01-31 14:33:27
