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lixingliang

新虫 (小有名气)

应助: 12 (小学生)
金币: 383.5
散金: 100
帖子: 181
在线: 41.6小时
虫号: 1254305
注册: 2011-04-03
性别: GG
专业: 生物化学

[求助] 紧急求助！如何回答reviewer重做实验的建议

时逢圣诞期间，以为老外休假，没想到今天收到主编回复，要求大修（所投杂志是Pesticide Biochemistry and Physiology ），和导师讨论后意见出现分歧，不知道该怎么办，向大家求助。
求助问题1：有两个reviewer回复意见，都提出语言需要改进，老板很坚决的说不会花钱找公司改文章，顶多找个native speaker免费修改，这是老板的决定，本人没办法说服花钱找公司改。
求助问题2：我的研究是寻找一种药物的作用靶点，其中一个reviewer提出药物处理浓度太高，以至于结果不可信，建议用LD50的40% and 80%的浓度重复试验。也就是说需要所有的实验重新做，老板也很坚决的说了，实验不会重复，也不会补数据，因为处理的药物和处理对象都没有了，不可能再做了。这可怎么办啊？如果不按照reviewer的建议以低浓度药物重复试验，会不会直接被拒？？
以上是主要的问题，其他的都是很小的问题。先粘贴editor回复如下：
Dear Dr. ###,
In regard to your manuscript ###," we now have the reviewer's comments. Based on these comments, we see that your manuscript needs Major revision. I would appreciate it if you could address these points in your revision. Please consider the reviewer's comments very carefully in revising your manuscript
and prepare a detailed "point-by-point" explanation of your revision/rebuttals. You are not required to follow every suggestion, but please provide your reasons for each rebuttal.
For your guidance, reviewers' comments are appended below.
Thank you for the opportunity to review your manuscript, and we look forward to receiving your revision.
John Marshall Clark, PhD
Pesticide Biochemistry and Physiology
Reviewers' comments:
Reviewer #1: This manuscript presents interesting results and provides useful methods for molluscicidal mechanism of A on Oncomelania hupensis. However, in my knowledge, the concentrations of A and Nic exposed to snails are too high to obtain the reliable results. The concentrations of 40% and 80% LD50 are much better for the study of molluscicidal mechanism. If possible, the authors should use the proper concentrations to repeat this study.
The paper requires a revision of the language and grammar which should be done with the assistance of someone who is more conversant with the language.
Reviewer #2:
The authors investigated the effect of A on snail mitochondria. The molluscicidal activity of Ais already well established. The present investigations now provide some additional data on the possible mode of action.
Generally, the paper is well prepared, the methods applied are appropriate and the conclusions drawn are conclusive.However, there are a number of points which need careful consideration before publication can be recommended:
*    The paper needs a very comprehensive revision with respect to English grammar and syntax.
*    Please write ginkgolic acids and niclosamide with a small initial letter throughout the manuscript
*    The investigations cited in reference [14] were conducted with LLC-MK(2) as well as HaCaT cells.
*    Page 7, line 1-2: Schistosomiasis joponica should be changed to S. japonica
*    Page 9: As described in "Statistical Analysis" correlation coefficients were calculated. However, no such results are given.
*    Page 9: Section 3.1 could entirely be deleted as most of these information are already given in the Materials and Methods section.
*    Page 10, line 4: LD50 values at 12 h are also shown in Tab. 2
*    Page 10, Tab. 2: What statistical test was applied for the calculation of p-values? The legend to Tab. 2 refers to superscripts which do not occur in the Table.
*    Page 11, line 3: According to Fig. 4 the expression of adenylate kinase does already decrease at 12 h while the mRNA level for cytochrome b seems to drop down only after 24 and 48 h. This disagreement needs to be solved.
*    Page 11, line 16-18. Unpublished work should not be cited.
*    Page 12, line 13: Expression of five mitochondrial enzymes was allegedly investigated. ?
*    Page 13: Citation of reference 10 is incomplete.
我们实验室的人都不容易毕业，原因吗，这个大家都懂得