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一北向路金虫 (小有名气)
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[求助]
各位大神帮帮忙啊！翻译这段英文啊！
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The fluorescence and UV absorption spectra were measured with a 850 CRT fluorescence spectrophotometer (Hitachi, Japan) and a Cary 50 probe UV–vis spectrophotometer (Varian, America), respectively. The fluorescence spectrophotometer was also used for the measurement of chemiluminescence spectrum. A Strata TM-X C18 column was used for the extraction of resveratrol. Effects of the reaction parameter on the CL intensity were investigated. The optimum conditions were 1 mol/L H2SO4, 0.025 mmol/L KMnO4, 0.56 mol/L HCHO, 3.5 mL/min of flow rate for P2 and 100 μL of sample loop, respectively. Under the selected optimum conditions, the calibration curves for the determination of resveratrol in red wine were established. Under the optimum conditions, the relative chemiluminescence intensity was linear with the concentration of resveratrol from 1.32 × 10−8 to 1.32 × 10−5 mol/L. The detection limit was 3.30 × 10−9 mol/L (3σ). The relative standard deviation for 11 parallel measurement of 1.32 × 10−5 mol/L resveratrol was 3.8%. The regression equation was ▵I = 10.52 + 0.19 × 108 C (C is the concentration of resveratrol, mol/L) with a correlation coefficient of 0.9994 (n = 11). The effects of foreign species on the determination of resveratrol were assessed. The tolerable limit of a foreign species was taken if it caused a relative error of less than 5%. The obtained results showed that under the optimized conditions, 1000-fold methanol; 500-fold maltose and fructose; 200-fold citrate, ethanol, lactose, glucose, K+, Na+, Zn2+, NH4+, Ni2+, Cr3+, Ca2+, Al3+, Cl− and 5-fold riboflavin did not affect the determination process. The proposed method was applied to determine resveratrol in red wine and the average concent of resveratrol in the analyzed red wine was 0.409 mg/L. The recovery experiment was also performed and the results obtained are given in Table 1. |
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13599388598
金虫 (正式写手)
- 翻译EPI: 17
- 应助: 1 (幼儿园)
- 金币: 911.1
- 散金: 300
- 红花: 2
- 帖子: 364
- 在线: 52.1小时
- 虫号: 1472756
- 注册: 2011-11-02
- 专业: 水产生物营养与饲料学
【答案】应助回帖
一北向路(金币+15, 翻译EPI+1): 2011-12-06 15:41:10
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分别通过850 CRT荧光光谱仪（日立，日本）和一个cary50 probe UV-vis光谱仪（varian，美国）检测荧光和UV吸收光谱。用荧光光谱仪测定化学发光光谱。一个strata TM-x C18柱被用于白藜芦醇的提取。 实验研究了CL反应强度。优化条件分别是1mol/L的硫酸，0.025mmol/L的高锰酸钾，0.56mol/L的HCHO，P2的流量为3.5mL/min和100uL的样品。在优化条件的选择下，我们建立了红葡萄酒中白藜芦醇的检测曲线。在优化条件选择下，我们采用浓度区间从1.32*10-8到1.32*10-5mol/L的白藜芦醇将相关化学发光强度线性化。其检测限量为3.3*10-9mol/L。11个平行对1.32*10-5mol/L的白藜芦醇进行检测的相关标准偏差为3.8%。回归方程为 ▵I = 10.52 + 0.19 × 108 C（C为白藜芦醇的浓度，mol/L)，其置信度为0.9994（n=11）。 对外来物种进行了白藜芦醇的检测。如果其产生了相关误差小于5%，外来物种的可以忍受的限定值【tolerable limit是否有专用翻译词小弟不知】可被采用。我们获得的结果表明优化的条件：1000倍甲醇；500倍的麦芽糖和果糖；200被的柠檬酸盐，乙醇，乳糖，葡萄糖，K+, Na+, Zn2+, NH4+, Ni2+, Cr3+, Ca2+, Al3+, Cl−和5倍的核黄素不会影响检测过程。 我们所建立的方法被用于检测红酒中的白藜芦醇，白藜芦醇的平均含量为0.409mg/L。我们同时也进行了恢复试验，其结果再表1中。 |
2楼2011-12-06 15:08:47