| 查看: 1615 | 回复: 5 | |||||
| 【奖励】 本帖被评价4次，作者modestmonkey增加金币 3.2 个 | |||||
[资源]
《柳叶刀》最新牛文 世界首例干细胞复合生物人工纳米气管支气管移植
|
|||||
|
《lancet》11月24日牛文：世界首例干细胞复合生物人工纳米气管支气管移植 Tracheobronchial transplantation with a stem-cell-seeded bioartificial nanocomposite: a proof-of-concept study SummaryBackground Tracheal tumours can be surgically resected but most are an inoperable size at the time of diagnosis; therefore, new therapeutic options are needed. We report the clinical transplantation of the tracheobronchial airway with a stem-cell-seeded bioartificial nanocomposite. Methods A 36-year-old male patient, previously treated with debulking surgery and radiation therapy, presented with recurrent primary cancer of the distal trachea and main bronchi. After complete tumour resection, the airway was replaced with a tailored bioartificial nanocomposite previously seeded with autologous bone-marrow mononuclear cells via a bioreactor for 36 h. Postoperative granulocyte colony-stimulating factor filgrastim (10 μg/kg) and epoetin beta (40 000 UI) were given over 14 days. We undertook flow cytometry, scanning electron microscopy, confocal microscopy epigenetics, multiplex, miRNA, and gene expression analyses. Findings We noted an extracellular matrix-like coating and proliferating cells including a CD105+ subpopulation in the scaffold after the reseeding and bioreactor process. There were no major complications, and the patient was asymptomatic and tumour free 5 months after transplantation. The bioartificial nanocomposite has patent anastomoses, lined with a vascularised neomucosa, and was partly covered by nearly healthy epithelium. Postoperatively, we detected a mobilisation of peripheral cells displaying increased mesenchymal stromal cell phenotype, and upregulation of epoetin receptors, antiapoptotic genes, and miR-34 and miR-449 biomarkers. These findings, together with increased levels of regenerative-associated plasma factors, strongly suggest stem-cell homing and cell-mediated wound repair, extracellular matrix remodelling, and neovascularisation of the graft. Interpretation Tailor-made bioartificial scaffolds can be used to replace complex airway defects. The bioreactor reseeding process and pharmacological-induced site-specific and graft-specific regeneration and tissue protection are key factors for successful clinical outcome. Funding European Commission, Knut and Alice Wallenberg Foundation, Swedish Research Council, StratRegen, Vinnova Foundation, Radiumhemmet, Clinigene EU Network of Excellence, Swedish Cancer Society, Centre for Biosciences (The Live Cell imaging Unit), and UCL Business. 原文链接：http://www.thelancet.com/journal ... 11)61715-7/fulltext |
回复此楼» 收录本帖的淘帖专辑推荐
 Nano&Micro | 纳米材料及生物材料前沿资源 | Point |
» 猜你喜欢
学科生物354求调剂
已经有0人回复
-
大连工业大学 生物质能源与材料专业招收调剂生，有兴趣加联系：15732893021
已经有0人回复
-
生物力学与组织工程学论文润色/翻译怎么收费?
已经有182人回复
-
生物光子学研究院/激光生命科学教育部重点实验室 研究生调剂
已经有0人回复
-
大连工业大学 生物质能源与材料专业招收调剂生，有兴趣加联系：15732893021
已经有0人回复
-
求调剂！生物与医药专硕
已经有5人回复
-
大连工业大学 生物质能源与材料专业招收调剂生，有兴趣加联系：15732893021
已经有0人回复
-
大连工业大学 生物质能源与材料专业招收调剂生，有兴趣将简历发送到507432328@qq.com
已经有0人回复
-
生物学308分求调剂（一志愿华东师大）
已经有7人回复
-
大连工业大学 生物质能源与材料专业招收调剂生，有兴趣将简历发送到507432328@qq.com
已经有0人回复
-
生物学308分求调剂（一志愿华东师大）
已经有10人回复
» 本主题相关商家推荐: (我也要在这里推广)
» 本主题相关价值贴推荐，对您同样有帮助:
- SERS又出牛文啦，非标记检测细菌！！！
已经有117人回复
- 能发牛文（不能中项目）的牛人VS能中项目（不能发好文章） 你选哪个？
已经有59人回复
- 【交流】虫子们写晶体文章都引用这篇千古牛文不:Acta Cryst. 2008, A64, 112-122
已经有9人回复
- 【交流】利用合成生物学方法研究青蒿素的合成nature牛文一篇
已经有12人回复
2楼2011-11-29 12:22:22
4楼2011-11-30 12:57:12
5楼2011-11-30 16:15:21
6楼2011-12-25 02:26:55
简单回复
2011-11-30 10:50
回复
五星好评 谢谢分享
10