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ral_hope木虫 (小有名气)
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[求助]
ThT binding assays相关的一句话
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Under standard conditions, a stock solution of the rabbit/human/bovine PrP in 6 M guanidine hydrochloride was diluted to a final concentration of 10 M and incubated at 37o C in PBS buffer (pH 7.0) containing 1 M guanidine hydrochloride and 3 M urea in the absence and presence of different concentrations of crowding agents with continuous shaking at 220 rpm, and samples (50 l) were diluted into PBS buffer containing 12.5 M ThT, giving a final volume of 2.5 ml. The fluorescence of ThT was excited at 450 nm with a slit-width of 7.5 nm and the emission was measured at 480 nm with a slit-width of 7.5 nm on an LS-55 luminescence spectrometer (PerkinElmer Life Sciences, Shelton, CT). 里面的方框，表示 “微” |
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