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yangjian8792金虫 (小有名气)
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Dear Mr. Yang: I write you in regards to manuscript # JFPP-06-11-0182 entitled "Isolation and Identification of Histamine-forming Bacteria from Frozen Storage of Raw Skipjack Tuna ( Eleotridae ) in China" which you submitted to the Journal of Food Processing and Preservation. In view of the criticisms of the reviewer(s) found at the bottom of this letter, your manuscript has been denied publication in the Journal of Food Processing and Preservation. Thank you for considering the Journal of Food Processing and Preservation for the publication of your research. I hope the outcome of this specific submission will not discourage you from the submission of future manuscripts. Sincerely, Dr. Y. Martin Lo Editor-in-Chief, Journal of Food Processing and Preservation ymlo@umd.edu Reviewer(s)' Comments to Author: Reviewer: 1 Comments to the Author In the submitted manuscript, authors claim to have isolated and identified histamine forming bacteria from frozen storage of raw skipjack tuna. The title is inappropriate: this is not a study about isolation and microbial characterization, but rather a study about the setting up of a method for histamine determination. This fact is even clearer in the section of conclusions, where authors said to have demonstrated that “the HPLC internal standard by dansylating with......etc.” In addition: • The aim of the work is not clear • Five samples were very few • Ten isolates were very few • How microbiological analyses were performed (APC)? what substrate? Temperature? Incubation times? • How was evaluated the effect of the pH on histamine forming bacteria? Authors said that bacterial growth and histamine formation in test TSBH were determined (how?) after incubation at 30°C for 36h (of what?). • As written, the paper appears very vague and confusing: it should be refocused stating clearly the aim and underlining the importance of the conducted research to the scientific field. The relevance of the present study is insufficiently explained and difficult to understand. • The section “Materials and methods” is too detailed in some paragraphs, but deficient in several important aspects (see before) • The section “Results and discussion” is very confusing In conclusion, the above mentioned problems make other minor details through the text of little relevance to be underlined. In my opinion, the paper is not apt for publication. Reviewer: 2 Comments to the Author Do the necessary revision as given |
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6楼2011-11-07 11:56:02
epirsgis
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3楼2011-11-07 10:13:23
wangth08
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4楼2011-11-07 10:17:42
yangjian8792
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Reviewer’s comment The paper explains about fairly good work on the occurrence of histamine and histamine forming bacteria in frozen-stored skipjack tuna from East China Sea which are subsequently used for canning. The work is presented in simple language but with substantial number of mistakes regarding grammar and framing sentences. There are minor technical mistakes also. Some of them are enlisted below L- 47 Remove full stop berofre (Motil & Scrimshaw, 1979; Taylor, 1986). Replace ‘can vary’ with ‘may vary’ L-49-51 That scobroid… may be revised as “The naturally occurring high content of free histidine in the muscle of scombroid fish is the cause of histamine fish poisoning. L-52 Full sentence may be deleted. L-70 Replace ‘Skipjack tuna can’ with ‘Canned skipjack tuna’ L-72 “However, histamine content ….’’ May be revised as “However, histamine has been encountered in excess of the standards which has been a hindrance to export.” L-73 Full sentence may be deleted. L- 78 ‘…effect of’ should be written as ‘effects of’. L-80 ‘….would be’ should be replaced with ‘could be’. L-87 ‘would be’ should be replaced with ‘was’ L-93 What is ‘aseptic bag’? define properly. L-103 ‘The water content was conducted’ should be written as ‘…determined’ and give reference of the method explained. L-111 Delete ‘respectively’. L-115 ‘..fortified’ should be written as ‘fortified with’ L-117 Delete ‘color’ L-134 “..according to” to be replaced with “in” L-136-137 “ .. and the concentration…Diaminoheptane were” should be re-written as ‘keeping the concentration ..” “which was …mother solution” to be replaced with “and this solution was used as stock solution”. L-138 “They….” Write the dilution ratio of stock solution (mother solution) per mL (of water/HCl ?) and not the dilution ratio of histamine and diaminoheptane for preparing working solution. L-142 After “20μl of 100 μg/mL” a comma is missing. L-146 ‘filtrated’ to be replaced with ‘filtered’ L-160-163 The first line bears an ambiguous meaning. The recovery method is not clear. The sections 2.5 and 2.6 as referred are not clearly visible in the text. Explain the method comprehensively. L-172 ‘the cell pellet was’ to be replaced with ‘the cell pellets were’ L-175 13000g should be 13000rpm L-178 After MgCl2 delete space before comma L-180 Delete space before TaKaRa after bracket L-206 ‘..are present’ re-write ‘are presented’ Temperature should be written without ° sign, e.g. 55°C as 55C. All ‘et al.’ should be italicized ‘et al.’ Table title and figure titles should be written in uppercases. References should be written I in style of the journal, e.g., all authors’ name should be in upper case., year along with author’s name, page no. at last, etc. Refer a paper of the journal and see the instructions to author. In the text, writing of reference is not proper, e.g. in L-47 (Motil & Scrimshaw, 1979; Taylor, 1986) should be written as (Motil and Scrimshaw 1979; Taylor 1986). Finally check grammar by using Word’s “Spelling and Grammar” botton. The paper is up to the mark and may be accepted with minor revision. |
5楼2011-11-07 10:18:08
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