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yungbonjut金虫 (小有名气)
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2.2.3 SEM图分析 MAC和Ag-MAC的扫描电镜图如图3、图4。在分别放大10000倍和5 000倍的条件下，可看到MAC表面吸附沉积了很多极小且分布均匀的银粒子。通过图3、图4中的刻度对比可以看出银粒子的粒径大多在500nm以下，可以判断为纳米级的银粒子。未固载脂肪酶的Ag-MAC和固载后的Ag-MAC的扫描电镜图如图5，由图5中的对比可以看出脂肪酶被很好的吸附固载在Ag-MAC的孔道表面；同时，酶的吸附点更趋向于纳米银粒子附着的地方，这可能是由于纳米银粒子的一些纳米、配位和强吸附力的特性，使得酶更容易团聚到它周围。 |
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wildwolf110
禁虫 (正式写手)
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sltmac(金币+1): 2011-11-04 15:33:03
yungbonjut(金币+35): 很好，谢谢 2011-11-08 09:36:04
sltmac(金币+1): 2011-11-04 15:33:03
yungbonjut(金币+35): 很好，谢谢 2011-11-08 09:36:04
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3楼2011-11-04 14:45:46
fjtony163
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sltmac(金币+1): 辛苦~ 2011-11-04 15:33:00
yungbonjut(金币+15): 谢谢 2011-11-08 09:36:10
sltmac(金币+1): 辛苦~ 2011-11-04 15:33:00
yungbonjut(金币+15): 谢谢 2011-11-08 09:36:10
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2.2.3 Analysis on SEM graph. The SEM graphs of MAC and Ag-MAC are Fig.3 and 4. Under condition of magnifying for 10000 times and 5000 times, on the surface of MAC, tiny and average distributed silver particle can be seen to be attached. Comparing the scale in Fig. 3 and 4, the silver particles are less than 500 nm in size and could be considered as level of nanometer. Fig. 5 is graph of non-immobilized lipase Ag-MAC and immobilized one and shows that lipase is well attached to surface of pore of Ag-MAC. Meanwhile, attached point of enzyme is closer to nanometer silver particle, because features of nanometer, coordination, and strong attachment of nanometer silver particle make enzyme easy to gather around it. |
2楼2011-11-04 14:20:11
xiaokaizi
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爱与雨下(金币+1): 2011-11-07 08:37:49
yungbonjut(金币+10, 翻译EPI+1): 比较生硬 2011-11-08 09:35:45
爱与雨下(金币+1): 2011-11-07 08:37:49
yungbonjut(金币+10, 翻译EPI+1): 比较生硬 2011-11-08 09:35:45
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2.2.3 The analysis of SEM graph. The MAC and Ag-MAC SEM graphs, Fig.3 and Fig.4, are magnified 10000 times and 5000 times individually. In these graphs, tiny silver particles are averagely distributed on the surface of MAC. Comparing the scale in Fig. 3 and Fig. 4, the particles could be determined as silver particles at nanometer level, for most particles’ diameter are below 500 nm in size. Fig. 5 is the compare of non-immobilized lipase of Ag-MAC and the immobilized one and this graph shows that lipase is well attached to the surface of Ag-MAC pore; meanwhile, the enzyme attached point is closer to that of nanometer silver particles , and maybe it is for the silver particle features, nanometer coordination, strong attachment, resulting in enzyme attracted to silver particles easily . 呵呵，看看吧， |
4楼2011-11-06 19:31:12
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