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niunaiping

铜虫 (初入文坛)

[交流] Angew. Chem. Int. Ed. 2006, 45,4562 – 4588关于FRET的一篇综述 已有1人参与

The use of F!rster or fluorescence resonance energy transfer (FRET)
as a spectroscopic technique has been in practice for over 50 years. A
search of ISI Web of Science with just the acronym “FRET” returns
more than 2300 citations from various areas such as structural elucidation
of biological molecules and their interactions, in vitro assays,
in vivo monitoring in cellular research, nucleic acid analysis, signal
transduction, light harvesting and metallic nanomaterials. The advent
of new classes of fluorophores including nanocrystals, nanoparticles,
polymers, and genetically encoded proteins, in conjunction with ever
more sophisticated equipment, has been vital in this development. This
review gives a critical overview of the major classes of fluorophore
materials that may act as donor, acceptor, or both in a FRET configuration.
We focus in particular on the benefits and limitations of these
materials and their combinations, as well as the available methods of
bioconjugation.[ Last edited by niunaiping on 2011-11-1 at 09:55 ]
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ke30ke

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好东西
顶一下
2楼2011-11-01 10:56:16
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