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ghostldz金虫 (小有名气)
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[求助]
求助把这段文字翻译成英文，谢谢啦
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| 本文合成了一种新型一氧化氮分子荧光探针并对其结构进行了表征，此荧光探针为连接有1,4,8,11-四氮杂环十四烷环胺化合物的荧光素-Cu(II)离子配合物，其本身无荧光，当与NO反应后，在λex=465nm光激发下产生荧光且最大发射波长λem =524nm。体系的pH值对荧光强度有影响，在pH为4.5时荧光强度最大。在H2O2，ONOO-存在下，荧光探针对NO表现出很好的选择性。以NOC-18为NO释放剂，在NOC-18浓度为4×10-5~14×10-5mol/L范围内建立了NOC-18浓度和荧光强度间的关系曲线，荧光强度随NOC-18浓度的增加而增大，可实现对NO的直接检测。 |
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【答案】应助回帖
★ ★ ★ ★
爱与雨下(金币+2): ！~ 2011-09-13 08:37:07
sltmac(金币+2): 辛苦~ 2011-09-15 08:02:58
ghostldz(金币+50, 翻译EPI+1): 谢谢啦 2011-09-15 22:21:12
爱与雨下(金币+2): ！~ 2011-09-13 08:37:07
sltmac(金币+2): 辛苦~ 2011-09-15 08:02:58
ghostldz(金币+50, 翻译EPI+1): 谢谢啦 2011-09-15 22:21:12
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Let me try it. I am not familiar with your field, just a try. Here, a novel fluorescent probe for nitric oxide was synthesized the structure was explored. The probe was coupled with cyclam-Cu2+ complex that it alone does not emit fluorescence. However, when reacted with NO, excited under the wavelength of 465nm, it will emit fluorescence and the maximum wavelength of emission is 524nm. The intensity of fluorescence was influenced by PH value and the intensity reached peak value under PH4.5. The fluorescent probe exhibited excellent selection to NO in the appearance of H2O2 and/or ONOO-. A relationship curve was developed between fluorescent intensity and NOC-18, a stimulator of NO production. At range of 4x10e5 to14x10e5 mol/L of NOC-18, the fluorescent intensity was positively regulated by increasing NOC-18 concentration. Hence, NO production can be determined directly through measuring fluorescent intensity. |
6楼2011-09-13 03:24:04
ghostldz
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3楼2011-09-10 23:09:56
生化道长
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4楼2011-09-10 23:29:30
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