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huojinlong9287银虫 (小有名气)
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[求助]
求助中文翻译为英文一段话
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| ***是世界上第一个培育成功的大型哺乳动物近交系，在开展异种器官移植、疾病动物模型、转基因动物、基因修饰、基因敲除及功能基因研究等方面具有巨大的应用价值。本研究拟针对***异种器官移植过程中与免疫排斥反应相关的新基因进行研究，通过采用数字基因表达谱方法进行高通量筛选基因，illumina测序，对测序结果评估，基因注释，EST比对，筛选并初步确定与免疫排斥反应相关的新基因。采用real-time PCR和Northern-blotting检测基因的表达水平，通过表达水平与免疫排斥反应的相关性分析，确定与排斥相关的关键功能基因，并进行多组织表达谱分析、功能预测和鉴定。为揭示异种移植中免疫排斥反应的相关研究奠定基础，也可为下一步进行基因修饰和基因敲除确定重要的目标靶基因，最终为提高***在异种器官移植领域中的成功率提供科学依据。 |
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yerufei_2010
新虫 (著名写手)
- 翻译EPI: 27
- 应助: 0 (幼儿园)
- 金币: 1.2
- 散金: 2528
- 红花: 8
- 帖子: 1802
- 在线: 163.4小时
- 虫号: 1181021
- 注册: 2010-12-31
- 性别: GG
- 专业: 金属有机化学
【答案】应助回帖
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爱与雨下(金币+1): ！！ 2011-09-06 20:26:13
huojinlong9287(金币+200, 翻译EPI+1): 2011-09-07 21:54:02
爱与雨下(金币+1): ！！ 2011-09-06 20:26:13
huojinlong9287(金币+200, 翻译EPI+1): 2011-09-07 21:54:02
| *** is the world's first successful cultivation of a large mammal inbred,playing a important role in carrying out xenotransplantation, animal models of disease, transgenic animals, genetically modified, gene knockout and functional genomics research .This research focuses on new genes related to immune rejection during *** xenotransplantation Process , through the use of digital gene expression profiling method ，the high-throughput screening gene and illumina sequencing were carried out, the sequencing results was evaluated, gene annotation, EST comparison, the new gene associated with immune rejection was screened and determined preliminary. Expression of Gene was detected by the means of real-time PCR and Northern-blotting,the key gene associated with immune rejection was determined through correlation analysis of expression level and immune rejection，and multi-tissue expression pattern analysis, function prediction and identification were carried out.It is not only lay the foundation for revealing xenograft rejection in immune research，but also for identifying further important target gene in conduct of genetic modification and gene knockout ,finally it provides a scientific basis for increase of the success rate in the field of xenotransplantation. |
2楼2011-09-06 20:08:11
