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Detecting Allergens in Food
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Contents
PART 1 THE BASICS OF FOOD ALLERGY
PART 2 TYPES OF DETECTION METHOD
PART 3 DETECTION METHODS FOR PARTICULAR ALLERGENS
PART 4 ISSUES IN USING ALLERGEN DETECTION METHODS

PART 1 THE BASICS OF FOOD ALLERGY
The nature of food allergy
S Taylor, University of Nebraska, USA
- Introduction: defining food allergy
- Mechanisms of food allergy
- Avoidance diets and treatment for IgE-mediated food allergies
- Future trends
- Sources of further information and advice
- References

Classifying food allergens
H Breiteneder, University of Vienna, Austria
- Introduction
- Plant food allergens
- Animal derived food allergens
- Future trends
- Sources of further information and advice
- References

PART 2 TYPES OF DETECTION METHOD
The use of antibodies to detect allergens in food
S Hefl¨¦, University of Nebraska, J Yeung, National Food Processors Association and R Helm, University of Arkansas, USA
- Introduction: the nature of antibodies
- Immunogens and antigens
- Antibody production
- Producing monoclonal or polyclonal antibodies
- References

Allergen-specific human IgE antibody-based analysis of food
R Hamilton, Johns Hopkins University School of Medicine, USA
- Introduction
- IgE antibody-based in vivo assay
- IgE antibody-based in vitro assay for food allergen detection
- Applications
- RAST inhibition assay strengths and weaknesses
- Future trends
- References

Immunoblotting in allergen detection
R van Ree, J Akkerdaas and L Zuidmeer, Sanquin Research, The Netherlands
- Introduction
- Mono-specific antibody reagents
- Critical assessment of mono-specificity
- Food processing and antibody specificity
- Future trends
- References

Enzyme-linked immunosorbent assays (ELISAs) for detecting allergens in foods
J Yeung, National Food Processors Association, USA
- Introduction
- Principles of ELISAs
- Applications
- Future trends
- Conclusions
- References

Polymerase chain reaction (PCR) methods for the detection of allergenic foods
T Holzhauser, O Stephan and S Vieths, Paul-Ehrlich-Institut, Germany
- Introduction
- PCR principles
- Application of PCR for the detection of allergenic foods
- Advantages and disadvantages of PCR compared to ELISA
- Future trends
- References

Proteomic assessment of allergens in food
M Zeece, J Markwell, G Sarath and X Gu, University of Nebraska, USA
- Introduction
- Key issues in proteomic assessment of allergens
- Applications of proteomics for detection of allergens
- Future trends
- Conclusions
- References

Detecting food allergens with a surface plasmon resonance immunoassay
H Jonsson and A Eriksson, Biacore AB and I Malmheden Yman, National Food Administration, Sweden
- Introduction
- Biosensors and SPR technology
- Developing a food allergen SPR immunoassay
- Published methods
- Experimental data
- Conclusions
- Sources of further information and advice
- References

The use of lateral flow devices to detect food allergens
R Verheijen and S Baumgartner, IFA-Tulln, Austria
- Introduction
- Antibodies
- Constructing a lateral flow device
- Running a sample
- Methods available
- Future trends
- References

PART 3 DETECTION METHODS FOR PARTICULAR ALLERGENS
Methods for detecting peanuts in food
S Hefl¨¦, University of Nebraska, USA
- Introduction: peanut allergy
- Allergenic peanut proteins
- Peanut detection methods
- Appropriate detection limits for peanut methods
- Future trends
- References

Detecting tree nuts and seeds in food
S Koppelman, TNO Nutrition and Food Research and Utrecht University Medical Centre, The Netherlands
- Introduction
- Prevalence of nut and seed allergies
- Thresholds
- Allergenic proteins in nuts and seeds
- Effect of food processing on allergenicity
- Detecting nut and seed residues in food: selecting a method
- Conclusions
- References

Detecting dairy and egg residues in food
C Demeulemester and V Leduc, Centre Technique de la Salaison, de la Charcuterie and des Conserves de Viandes (CTSCCV) and I Giovannacci, Allerbio, France
- Introduction
- Milk
- Egg
- Types of detection methods
- Future trends
- References

Detecting wheat gluten in food
F Janssen, Fascoda FAS Food Consultancy, The Netherlands
- Introduction
- Key requirements for detection and quantisation
- Types of detection methods
- Non-antibody-based techniques
- Selecting a method
- Future trends
- Sources of further information and advice
- References

Detecting soy, fish and crustaceans in food
S Koppelman, TNO Nutrition and Food Research and Utrecht University Medical Centre, The Netherlands and S. Hefl¨¦, University of Nebraska, USA
- Introduction
- Soy
- Crustaceans
- Fish
- Future trends
- References

PART 4 ISSUES IN USING ALLERGEN DETECTION METHODS
Allergen quality assurance for hypoallergenic formula
C Cordle, Abbott Laboratories, USA
- Introduction
- Key terms and clinical and analytical performance targets
- Analytical methods
- Applications
- Summary and future trends
- References

Common issues in detecting allergenic residues on equipment and in processed foods
R Crevel, Unilever R&D Colworth, UK
- Introduction
- Food allergy and product safety
- Management of food allergy risks
- Role of allergen detection and other considerations
- Future trends
- References

Factors affecting the effectiveness of allergen detection
U Immer, R-Biopharm AG, Germany
- Introduction
- Factors affecting the determination of allergenic residues
- Troubleshooting
- Future trends
- Summary
- References

Reference materials and method validation in allergen detection
R Poms, International Association for Cereal Science and Technology, Austria, H Emons and E Anklam, Institute for Reference Materials and Measurements, Belgium
- Introduction
- Quality assurance for the analysis of allergens
- Towards validated methods for allergen determination
- Characteristics and use of reference materials
- Towards reference materials for allergens
- References

US regulation of undeclared allergens in food products
M Hahn, Hogan & Hartson LLP, USA
- Introduction
- Regulatory liability
- Legal grounds for product liability actions
- Future trends
- Conclusion
- Sources of further information and advice
- References
- Appendix I: summary of cases involving known allergens
- Appendix II: summary of cases involving ingredients that are not allergens

EU regulation of undeclared allergens in food products
H Heeres, TNO Quality of Life, The Netherlands
- Introduction
- Food legislation concerning the labelling of ingredients
- Food legislation concerning the labelling of allergens
- Legislation concerning general product safety (Directive 2001/95/EC)
- Legislation concerning food safety (Regulation (EC) No
- 2002/178/EC)
- Legislation concerning product liability
- Key issues in labelling of allergens, undeclared allergens, food safety and product liability
- Future trends
- Sources of further information and advice
- References

[ Last edited by signal023 on 2008-5-15 at 17:23 ]
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