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Detecting Allergens in Food ʳƷ¹ýÃôÔ´¼ì²â Contents PART 1 THE BASICS OF FOOD ALLERGY PART 2 TYPES OF DETECTION METHOD PART 3 DETECTION METHODS FOR PARTICULAR ALLERGENS PART 4 ISSUES IN USING ALLERGEN DETECTION METHODS PART 1 THE BASICS OF FOOD ALLERGY The nature of food allergy S Taylor, University of Nebraska, USA - Introduction: defining food allergy - Mechanisms of food allergy - Avoidance diets and treatment for IgE-mediated food allergies - Future trends - Sources of further information and advice - References Classifying food allergens H Breiteneder, University of Vienna, Austria - Introduction - Plant food allergens - Animal derived food allergens - Future trends - Sources of further information and advice - References PART 2 TYPES OF DETECTION METHOD The use of antibodies to detect allergens in food S Hefl¨¦, University of Nebraska, J Yeung, National Food Processors Association and R Helm, University of Arkansas, USA - Introduction: the nature of antibodies - Immunogens and antigens - Antibody production - Producing monoclonal or polyclonal antibodies - References Allergen-specific human IgE antibody-based analysis of food R Hamilton, Johns Hopkins University School of Medicine, USA - Introduction - IgE antibody-based in vivo assay - IgE antibody-based in vitro assay for food allergen detection - Applications - RAST inhibition assay strengths and weaknesses - Future trends - References Immunoblotting in allergen detection R van Ree, J Akkerdaas and L Zuidmeer, Sanquin Research, The Netherlands - Introduction - Mono-specific antibody reagents - Critical assessment of mono-specificity - Food processing and antibody specificity - Future trends - References Enzyme-linked immunosorbent assays (ELISAs) for detecting allergens in foods J Yeung, National Food Processors Association, USA - Introduction - Principles of ELISAs - Applications - Future trends - Conclusions - References Polymerase chain reaction (PCR) methods for the detection of allergenic foods T Holzhauser, O Stephan and S Vieths, Paul-Ehrlich-Institut, Germany - Introduction - PCR principles - Application of PCR for the detection of allergenic foods - Advantages and disadvantages of PCR compared to ELISA - Future trends - References Proteomic assessment of allergens in food M Zeece, J Markwell, G Sarath and X Gu, University of Nebraska, USA - Introduction - Key issues in proteomic assessment of allergens - Applications of proteomics for detection of allergens - Future trends - Conclusions - References Detecting food allergens with a surface plasmon resonance immunoassay H Jonsson and A Eriksson, Biacore AB and I Malmheden Yman, National Food Administration, Sweden - Introduction - Biosensors and SPR technology - Developing a food allergen SPR immunoassay - Published methods - Experimental data - Conclusions - Sources of further information and advice - References The use of lateral flow devices to detect food allergens R Verheijen and S Baumgartner, IFA-Tulln, Austria - Introduction - Antibodies - Constructing a lateral flow device - Running a sample - Methods available - Future trends - References PART 3 DETECTION METHODS FOR PARTICULAR ALLERGENS Methods for detecting peanuts in food S Hefl¨¦, University of Nebraska, USA - Introduction: peanut allergy - Allergenic peanut proteins - Peanut detection methods - Appropriate detection limits for peanut methods - Future trends - References Detecting tree nuts and seeds in food S Koppelman, TNO Nutrition and Food Research and Utrecht University Medical Centre, The Netherlands - Introduction - Prevalence of nut and seed allergies - Thresholds - Allergenic proteins in nuts and seeds - Effect of food processing on allergenicity - Detecting nut and seed residues in food: selecting a method - Conclusions - References Detecting dairy and egg residues in food C Demeulemester and V Leduc, Centre Technique de la Salaison, de la Charcuterie and des Conserves de Viandes (CTSCCV) and I Giovannacci, Allerbio, France - Introduction - Milk - Egg - Types of detection methods - Future trends - References Detecting wheat gluten in food F Janssen, Fascoda FAS Food Consultancy, The Netherlands - Introduction - Key requirements for detection and quantisation - Types of detection methods - Non-antibody-based techniques - Selecting a method - Future trends - Sources of further information and advice - References Detecting soy, fish and crustaceans in food S Koppelman, TNO Nutrition and Food Research and Utrecht University Medical Centre, The Netherlands and S. Hefl¨¦, University of Nebraska, USA - Introduction - Soy - Crustaceans - Fish - Future trends - References PART 4 ISSUES IN USING ALLERGEN DETECTION METHODS Allergen quality assurance for hypoallergenic formula C Cordle, Abbott Laboratories, USA - Introduction - Key terms and clinical and analytical performance targets - Analytical methods - Applications - Summary and future trends - References Common issues in detecting allergenic residues on equipment and in processed foods R Crevel, Unilever R&D Colworth, UK - Introduction - Food allergy and product safety - Management of food allergy risks - Role of allergen detection and other considerations - Future trends - References Factors affecting the effectiveness of allergen detection U Immer, R-Biopharm AG, Germany - Introduction - Factors affecting the determination of allergenic residues - Troubleshooting - Future trends - Summary - References Reference materials and method validation in allergen detection R Poms, International Association for Cereal Science and Technology, Austria, H Emons and E Anklam, Institute for Reference Materials and Measurements, Belgium - Introduction - Quality assurance for the analysis of allergens - Towards validated methods for allergen determination - Characteristics and use of reference materials - Towards reference materials for allergens - References US regulation of undeclared allergens in food products M Hahn, Hogan & Hartson LLP, USA - Introduction - Regulatory liability - Legal grounds for product liability actions - Future trends - Conclusion - Sources of further information and advice - References - Appendix I: summary of cases involving known allergens - Appendix II: summary of cases involving ingredients that are not allergens EU regulation of undeclared allergens in food products H Heeres, TNO Quality of Life, The Netherlands - Introduction - Food legislation concerning the labelling of ingredients - Food legislation concerning the labelling of allergens - Legislation concerning general product safety (Directive 2001/95/EC) - Legislation concerning food safety (Regulation (EC) No - 2002/178/EC) - Legislation concerning product liability - Key issues in labelling of allergens, undeclared allergens, food safety and product liability - Future trends - Sources of further information and advice - References [ Last edited by signal023 on 2008-5-15 at 17:23 ] |
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