²é¿´: 3099  |  »Ø¸´: 35

Î÷¹Ï

ÈÙÓþ°æÖ÷ (ÖªÃû×÷¼Ò)

[½»Á÷] ·Ö×ÓÉúÎï°æ¡¾Journal Club¡¿Ô¤¸æ£º¡ïµÚ1½²¡ï8ÔÂ16ÈÕ:´ÅÐÔÄÉÃײÄÁÏÓÃÓÚºËËáµÄ·ÖÀë´¿»¯ ÒÑÓÐ25È˲ÎÓë

µÚ1½²ÒѾ­Õýʽ¿ª½²£¬ÇëÒÆ²½£ºhttp://muchong.com/bbs/viewthread.php?tid=3490537&fpage=1
ÓÐÐËȤµÄ³æ×ӿɵ½»î¶¯Ö÷Ìù¸úÌû±¨Ãû£ºhttp://muchong.com/bbs/viewthread.php?tid=3468054&fpage=0&view=&highlight=&page=1

Contributions of Phosphate to DNA Adsorption/Desorption Behaviors
on Aminosilane-Modified Magnetic Nanoparticles

Tanaka T, Sakai R, Kobayashi R, Hatakeyama K, Matsunaga T
Langmuir. 2009 Mar 3;25(5):2956-61.

¡¾Ö÷³ÖÈË¡¿£º

¡¾¿ª½²Ê±¼ä¡¿£º2011Äê8ÔÂ16ÈÕ 19:00
¡¾ÄÚÈݼò½é¡¿£ºÖ÷Òª¸ø´ó¼Ò½éÉÜÏ´øÓа±»ù»ùÍŵĴÅÐÔÄÉÃײÄÁÏÓÃÓÚºËËáµÄ·ÖÀë´¿»¯¡£½ìʱÖ÷³ÖÈ˽«ÔÚ·Ö×ÓÉúÎï°æ¿ªÌù¸ø´ó¼Ò½²½â£¬»¶Ó­´ó¼Òµ½Ê±»ØÌû½»Á÷£¬Ö÷³ÖÈËÒ»ÖÜÖ®ÄÚÿÌì¶¼»á³éʱ¼ä»ØÌû¡£
Abstract£º The adsorption and desorption behaviors of DNA on aminosilane-modified magnetic nanoparticles were investigated by altering both type of anions and solvation state to achieve eficient recovery of DNA useful for subsequent polymerase chain reaction (PCR) analysis. The effects of multiple anions in accordance with the Hofmeister ion series were determined to clarify the contribution of phosphate ions on the effective desorption of DNA from aminosilane surfaces. Efficient DNA desorption (85% recovery) occurred in the presence of 1 M phosphate buffer, however, little DNA desorption was observed using any other anions. This phenomenon indicates that desorption originated from the replacement of DNA by phosphate ions. Furthermore, the adsorption and desorption were significantly affected by the addition of both protic and aprotic solvents. Efficient recovery of adsorbed DNA was attained using deoxynucleotide triphosphates (dNTPs) in place of phosphate buffer and was suitable for subsequent PCR analyses. Therefore, the DNA adsorption/desorption process proposed in this study will be a promising, novel approach for DNA purification with a high recovery ratio that is suitable for subsequent enzymatic reactions, such as PCR or restriction enzyme digestion.[ Last edited by Î÷¹Ï on 2011-8-16 at 18:35 ]
»Ø¸´´ËÂ¥

» ±¾ÌûÒÑ»ñµÃµÄºì»¨£¨×îÐÂ10¶ä£©

» ±¾Ìû@֪ͨ

» ²ÂÄãϲ»¶

» ±¾Ö÷ÌâÏà¹ØÉ̼ÒÍÆ¼ö: (ÎÒÒ²ÒªÔÚÕâÀïÍÆ¹ã)

ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

1949stone

ÈÙÓþ°æÖ÷ (ÖªÃû×÷¼Ò)

º£Äɰٴ¨

Ö§³Ö ÎÒÊÇɳ·¢
º£Äɰٴ¨Ö¹ÓÚÖÁÉÆ
2Â¥2011-08-12 19:11:32
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

dhd997

ÈÙÓþ°æÖ÷ (ÎÄ̳¾«Ó¢)

×ÔÓÉÈË£¬ÎÒÐÍÎÒÐ㣡

ÓÅÐã°æÖ÷

ÈËÉú²»¹ý¼¸Ê®Ä꣬³É°ÜÈÙÈè¶¼ÔÚÌ죬ÊǷǶ÷ԹĪÔÚÒ⣬½¡¿µ¿ìÀÖ×îֵǮ£¬×Ô¹ÅÈ˼ä¿àÎޱߣ¬¿´µÃ¸ßÔ¶¾³ÈçÏÉ£¬Ô¸Ä㲻Ϊ·²ÊÂÄÕ£¬ÇáËÉ¿ìÀÖÿһÌì¡£
3Â¥2011-08-12 19:13:46
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

Î÷¹Ï

ÈÙÓþ°æÖ÷ (ÖªÃû×÷¼Ò)

ºÇºÇ£¬¶þλǰ±²ºÜ¸øÁ¦°¡£¡£¡
4Â¥2011-08-12 19:16:37
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

rainwander

Ìú¸Ëľ³æ (ÖªÃû×÷¼Ò)

ÓÅÐã°æÖ÷

ºÃ»î¶¯
ǧÍò²»ÒªÒòΪ×ßµÄÌ«¾Ã£¬¶øÍü¼ÇÁËÎÒÃÇΪʲô³ö·¢~~~~~~ForDream~~~~~~
5Â¥2011-08-12 20:44:13
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

¿Õ¹ÈÓÄ·ç

½ð³æ (ÕýʽдÊÖ)

¡ï
Сľ³æ(½ð±Ò+0.5):¸ø¸öºì°ü£¬Ð»Ð»»ØÌû
ºÜÆÚ´ýŶ£¬Ð»Ð»ÄãµÄÖ§³Ö£¡
a?,c??ae~?a,EURc¡ì?c?¦Ì¨¦-,cs,,a¡®?¨¦?¡°i1/4?i1/4?i1/4?i1/4?
6Â¥2011-08-12 20:58:32
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

whb8161

ľ³æ (СÓÐÃûÆø)

¡ï
Сľ³æ(½ð±Ò+0.5):¸ø¸öºì°ü£¬Ð»Ð»»ØÌû
ºÃ»î¶¯£¬ÒªÖ§³Ö£¡
ÈËÉú²»Ö¹¼Åį²»ÒÑ
7Â¥2011-08-12 21:32:22
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

yesucao

ľ³æ (ÕýʽдÊÖ)

Ö§³Ö£¬Ñ§Ï°£¡£¡
8Â¥2011-08-12 21:40:44
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

bluekinghuang

ľ³æ (ÕýʽдÊÖ)

ʱÕäÃÅͽ

¡ï
Сľ³æ(½ð±Ò+0.5):¸ø¸öºì°ü£¬Ð»Ð»»ØÌû
½ñÌìÌýÁËÒ»¸ö±¨¸æ£¬ÓÃÕâÖÖ²ÄÁÏÓÃÓÚ»îÐԳɷֵķÖÀë¡£
Ö§³Ö¸Ãм¼ÊõÓÃÓÚ·ÖÀë´¿»¯

» ±¾ÌûÒÑ»ñµÃµÄºì»¨£¨×îÐÂ10¶ä£©

×öÑо¿Òª×øµÃסÀä°åµÊ¡£
9Â¥2011-08-12 21:43:30
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

Î÷¹Ï

ÈÙÓþ°æÖ÷ (ÖªÃû×÷¼Ò)

ËÍÏÊ»¨Ò»¶ä
ÒýÓûØÌû:
9Â¥: Originally posted by bluekinghuang at 2011-08-12 21:43:30:
½ñÌìÌýÁËÒ»¸ö±¨¸æ£¬ÓÃÕâÖÖ²ÄÁÏÓÃÓÚ»îÐԳɷֵķÖÀë¡£
Ö§³Ö¸Ãм¼ÊõÓÃÓÚ·ÖÀë´¿»¯

ºÇºÇ£¬ÄÇ8ÔÂ16ÈÕÕýʽ¿ª½²µÄʱºòÄúÒ»¶¨À´°¡£¡ÆÚ´ýÄúµÄ¸ß¼û£¡
10Â¥2011-08-12 21:46:21
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
Ïà¹Ø°æ¿éÌø×ª ÎÒÒª¶©ÔÄÂ¥Ö÷ Î÷¹Ï µÄÖ÷Ìâ¸üÐÂ
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼ÑÐ] Ò»Ö¾Ô¸Î÷ÄϽ»Í¨ ר˶ ²ÄÁÏ355 ±¾¿ÆË«·Ç Çóµ÷¼Á +4 Î÷ÄϽ»Í¨×¨²Ä355 2026-03-19 4/200 2026-03-20 11:39 by »¨¿ª¸»¹óÐÒ¸£ÈËÉ
[¿¼ÑÐ] Ò»Ö¾Ô¸Î人Àí¹¤²ÄÁϹ¤³Ìר˶µ÷¼Á +7 Doleres 2026-03-19 7/350 2026-03-20 10:39 by EBSD
[¿¼ÑÐ] 0703»¯Ñ§µ÷¼Á £¬Áù¼¶Òѹý£¬ÓпÆÑо­Àú +12 êØÎõÙâ 2026-03-15 12/600 2026-03-19 19:42 by maocaozhuxi
[¿¼ÑÐ] ¸´ÊÔµ÷¼Á +4 z1z2z3879 2026-03-14 6/300 2026-03-19 17:18 by fei626-918
[¿¼ÑÐ] Ò»Ö¾Ô¸¸£´ó288Óлú»¯Ñ§£¬Çóµ÷¼Á +3 Сľ³æ200408204 2026-03-18 3/150 2026-03-19 13:31 by houyaoxu
[¿¼ÑÐ] Ò»Ö¾Ô¸Ìì´ó²ÄÁÏÓ뻯¹¤£¨085600£©×Ü·Ö338 +5 ²Ì´óÃÀÅ® 2026-03-13 5/250 2026-03-19 10:44 by ÊÇСÁõѽ¡«
[¿¼ÑÐ] Ò»Ö¾Ô¸985£¬±¾¿Æ211£¬0817»¯Ñ§¹¤³ÌÓë¼¼Êõ319Çóµ÷¼Á +10 Liwangman 2026-03-15 10/500 2026-03-19 10:25 by Î޼ʵIJÝÔ­
[¿¼ÑÐ] 0817µ÷¼Á +3 ûÓдð°¸_ 2026-03-14 3/150 2026-03-19 09:51 by Xu de nuo
[¿¼ÑÐ] 344Çóµ÷¼Á +6 knight344 2026-03-16 7/350 2026-03-18 20:13 by walc
[¿¼ÑÐ] 070300»¯Ñ§319Çóµ÷¼Á +6 ½õÀð0909 2026-03-17 6/300 2026-03-18 13:22 by Iveryant
[»ù½ðÉêÇë] ±»ÎÒÑÔÖУºÐÂÄ£°å²»Ç¿µ÷¸ñʽÁË£¬¼Ùר¼Ò¿ªÊ¼¹Ü¸ñʽÁË +4 beefly 2026-03-14 4/200 2026-03-17 22:04 by »ÆÄñÓÚ·ÉChao
[¿¼ÑÐ] ¿¼Ñл¯Ñ§Ñ§Ë¶µ÷¼Á£¬Ò»Ö¾Ô¸985 +4 ÕÅvvvv 2026-03-15 6/300 2026-03-17 17:15 by ruiyingmiao
[¿¼ÑÐ] 274Çóµ÷¼Á +5 ʱ¼äµã 2026-03-13 5/250 2026-03-17 07:34 by ÈÈÇéɳĮ
[¿¼ÑÐ] [µ¼Ê¦ÍƼö]Î÷ÄϿƼ¼´óѧ¹ú·À/²ÄÁϵ¼Ê¦ÍƼö +3 ¼â½ÇСºÉ 2026-03-16 6/300 2026-03-16 23:21 by ¼â½ÇСºÉ
[¿¼ÑÐ] 326Çóµ÷¼Á +4 ŵ±´¶û»¯Ñ§½±êéê 2026-03-15 7/350 2026-03-16 17:11 by ŵ±´¶û»¯Ñ§½±êéê
[¿¼ÑÐ] Ò»Ö¾Ô¸211 0703·½Ïò310·ÖÇóµ÷¼Á +3 ŬÁ¦·Ü¶·112 2026-03-15 3/150 2026-03-16 16:44 by houyaoxu
[¿¼ÑÐ] 326Çóµ÷¼Á +3 mlpqaz03 2026-03-15 3/150 2026-03-16 07:33 by Iveryant
[¿¼ÑÐ] 085601²ÄÁϹ¤³Ì315·ÖÇóµ÷¼Á +3 yang_0104 2026-03-15 3/150 2026-03-15 10:58 by peike
[¿¼ÑÐ] Öпƴó²ÄÁÏÓ뻯¹¤319Çóµ÷¼Á +3 ÃÏöβÄÁÏ 2026-03-14 3/150 2026-03-14 20:10 by ms629
[¿¼ÑÐ] ÕÐÊÕ0805£¨²ÄÁÏ£©µ÷¼Á +3 18595523086 2026-03-13 3/150 2026-03-14 00:33 by 123%¡¢
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û