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[交流] 分子生物版【Journal Club】预告：★第1讲★8月16日:磁性纳米材料用于核酸的分离纯化已有25人参与

第1讲已经正式开讲，请移步：http://muchong.com/bbs/viewthread.php?tid=3490537&fpage=1
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Contributions of Phosphate to DNA Adsorption/Desorption Behaviors
on Aminosilane-Modified Magnetic Nanoparticles

Tanaka T, Sakai R, Kobayashi R, Hatakeyama K, Matsunaga T
Langmuir. 2009 Mar 3;25(5):2956-61.

【主持人】：

【开讲时间】：2011年8月16日 19:00
【内容简介】：主要给大家介绍下带有氨基基团的磁性纳米材料用于核酸的分离纯化。届时主持人将在分子生物版开贴给大家讲解，欢迎大家到时回帖交流，主持人一周之内每天都会抽时间回帖。
Abstract： The adsorption and desorption behaviors of DNA on aminosilane-modified magnetic nanoparticles were investigated by altering both type of anions and solvation state to achieve eficient recovery of DNA useful for subsequent polymerase chain reaction (PCR) analysis. The effects of multiple anions in accordance with the Hofmeister ion series were determined to clarify the contribution of phosphate ions on the effective desorption of DNA from aminosilane surfaces. Efficient DNA desorption (85% recovery) occurred in the presence of 1 M phosphate buffer, however, little DNA desorption was observed using any other anions. This phenomenon indicates that desorption originated from the replacement of DNA by phosphate ions. Furthermore, the adsorption and desorption were significantly affected by the addition of both protic and aprotic solvents. Efficient recovery of adsorbed DNA was attained using deoxynucleotide triphosphates (dNTPs) in place of phosphate buffer and was suitable for subsequent PCR analyses. Therefore, the DNA adsorption/desorption process proposed in this study will be a promising, novel approach for DNA purification with a high recovery ratio that is suitable for subsequent enzymatic reactions, such as PCR or restriction enzyme digestion.[ Last edited by 西瓜 on 2011-8-16 at 18:35 ]

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