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小木虫论坛-学术科研互动平台 » 生物医药区 » 微生物 » 实验/技术 » （图）这是什么仪器？
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jayzhao520

木虫 (正式写手)

[交流] （图）这是什么仪器？ 已有2人参与

这是我跟文献作者要的一个 Sterile stirred batch culture fermentation vessels的图片，不知道到底是啥，推测是Electrolab这个公司的生物反应器
下面是文献里涉及的一部分内容：
2.3. Faecal batch culture fermentation
The fermentation profile of the freeze dried digested different
sized oat flakes, the prebiotic oligofructose (Raftilose P95 ORAFTI)
and the lowly fermented control carbohydrate cellulose (OXOID)
was determined using anaerobic, pH controlled faecal batch
cultures. Sterile stirred batch culture fermentation vessels (200 mL
working volume) were prepared and aseptically filled with 180 mL
of sterile basal nutrientmedium. Basalmedium contained per litre:
2 g Peptone, 2 g Yeast extract, 0.1 g NaCl, 0.04 g K2HPO4, 0.04 g
KH2PO4, 0.01 g MgSO47H20, 0.01 g CaCl26H2O, 2 g NaHCO3,2ml
Tween 80, 0.05 g Hemin dissolved in 1 ml of 4 M NaOH 10 ml
Vitamen K (SIGMA), 0.5 g L-Cysteine HCL, and 0.5 g Bile Salts
(sodium glycocholate and sodium taurocholate). The medium was
adjusted to pH 7.0 and 4 mL of 0.025% (w/v) resazurin solution
added prior to autoclaving. Once in the fermentation vessels, the
sterilemediumwas sparged with O2-free N2 (15mL/min) overnight
to maintain anaerobic conditions. The following day test substrates
were allowed to dissolve in the basal medium to give a final
concentration of 1% (w/v) before inoculation of the vesselswith 10%
(w/v) faecal slurry, which was prepared with pre-reduced sterile
phosphate-buffered saline (PBS, pH 7.0). Faecal samples were
collected fromthree healthy faecal donors. All donors were healthy
males aged between 23 and 31, and had not received antibiotic
treatment for at least 3 months prior to stool collection, had not
consumed pre- or probiotic supplements immediately prior to
experimentation, and had no history of bowel disorders. The temperature of the fermentation vessels was held at 37 C by use of
a circulating water bath, pH values were held between 6.7 and 6.8
by the addition of 0.5 M NaOH or HCl to the vessels, pH was
controlled via pH meter controllers (Electrolab260, UK) and
anaerobic conditions weremaintained by sparging the vessels with
O2-free N2 (15 mL/min). A 5 ml sample was taken from each vessel
immediately for analysis similarly samples were taken at 5, 10, and
24 h for analysis of SCFA by gas chromatography (GC) and for
analysis of bacterial populations by fluorescence in situ hybrid-
isation (FISH). This experiment was performed in triplicate.
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1楼 2011-08-10 15:07:00
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ganchao1776

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灌好买，控制不好弄
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5楼2011-08-17 23:54:13
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longage.gene

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amisking(金币+2): 鼓励应助 2011-08-10 20:15:52
通俗的硕就是桌上型发酵罐。作者是靠通N2来厌氧培养，还可以自动control PH。但看图片好像罐与罐之间是相通的。没看到文中说明为什么连接。也许是我英文不好，没看懂。也有可能相通的是循环水，文中说有循环水，但很难想像会5个一起连，这样最后的罐冷却就。。。。。。
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2楼2011-08-10 16:27:23
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jayzhao520

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引用回帖:
2楼: Originally posted by longage.gene at 2011-08-10 16:27:23:
通俗的硕就是桌上型发酵罐。作者是靠通N2来厌氧培养，还可以自动control PH。但看图片好像罐与罐之间是相通的。没看到文中说明为什么连接。也许是我英文不好，没看懂。也有可能相通的是循环水，文中说有循环水，但 ...

看起来是这样的，请问这种罐有没有地方可以买到
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3楼2011-08-12 12:46:32
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longage.gene

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我觉得这种罐国内应该有吧，又不是什么很难的东东。记得在国内我也用过类式的。
我这边是很容易买的到的。
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4楼2011-08-13 22:57:30
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