| 查看: 1402 | 回复: 4 | |||
jayzhao520木虫 (正式写手)
|
[交流]
（图）这是什么仪器？ 已有2人参与
|
|
这是我跟文献作者要的一个 Sterile stirred batch culture fermentation vessels的图片，不知道到底是啥，推测是Electrolab这个公司的生物反应器 下面是文献里涉及的一部分内容： 2.3. Faecal batch culture fermentation The fermentation profile of the freeze dried digested different sized oat flakes, the prebiotic oligofructose (Raftilose P95 ORAFTI) and the lowly fermented control carbohydrate cellulose (OXOID) was determined using anaerobic, pH controlled faecal batch cultures. Sterile stirred batch culture fermentation vessels (200 mL working volume) were prepared and aseptically filled with 180 mL of sterile basal nutrientmedium. Basalmedium contained per litre: 2 g Peptone, 2 g Yeast extract, 0.1 g NaCl, 0.04 g K2HPO4, 0.04 g KH2PO4, 0.01 g MgSO47H20, 0.01 g CaCl26H2O, 2 g NaHCO3,2ml Tween 80, 0.05 g Hemin dissolved in 1 ml of 4 M NaOH 10 ml Vitamen K (SIGMA), 0.5 g L-Cysteine HCL, and 0.5 g Bile Salts (sodium glycocholate and sodium taurocholate). The medium was adjusted to pH 7.0 and 4 mL of 0.025% (w/v) resazurin solution added prior to autoclaving. Once in the fermentation vessels, the sterilemediumwas sparged with O2-free N2 (15mL/min) overnight to maintain anaerobic conditions. The following day test substrates were allowed to dissolve in the basal medium to give a final concentration of 1% (w/v) before inoculation of the vesselswith 10% (w/v) faecal slurry, which was prepared with pre-reduced sterile phosphate-buffered saline (PBS, pH 7.0). Faecal samples were collected fromthree healthy faecal donors. All donors were healthy males aged between 23 and 31, and had not received antibiotic treatment for at least 3 months prior to stool collection, had not consumed pre- or probiotic supplements immediately prior to experimentation, and had no history of bowel disorders. The temperature of the fermentation vessels was held at 37 C by use of a circulating water bath, pH values were held between 6.7 and 6.8 by the addition of 0.5 M NaOH or HCl to the vessels, pH was controlled via pH meter controllers (Electrolab260, UK) and anaerobic conditions weremaintained by sparging the vessels with O2-free N2 (15 mL/min). A 5 ml sample was taken from each vessel immediately for analysis similarly samples were taken at 5, 10, and 24 h for analysis of SCFA by gas chromatography (GC) and for analysis of bacterial populations by fluorescence in situ hybrid- isation (FISH). This experiment was performed in triplicate. 求大神解答  |
回复此楼» 猜你喜欢
售T0P一区SCI文章，我:8O5.51.O.54,科目齐全,可＋急
已经有3人回复
-
售T0P一区SCI文章，我:8O5.51.O.54,科目齐全,可＋急
已经有3人回复
-
售T0P一区SCI文章，我:8O5.51.O.54,科目齐全,可＋急
已经有3人回复
-
售T0P一区SCI文章，我:8O5.51.O.54,科目齐全,可＋急
已经有4人回复
-
售T0P一区SCI文章，我:8O5.51.O.54,科目齐全,可＋急
已经有4人回复
-
b口会评
已经有6人回复
-
信息学部会评时间
已经有3人回复
-
祈祷青基必中
已经有9人回复
-
在职考研
已经有7人回复
-
MSER送审了还被拒稿
已经有5人回复
» 本主题相关价值贴推荐，对您同样有帮助:
- 求教实验高手，下面的仪器学名是什么？
已经有10人回复
- 我的仪器的电流表，表下面写个200比五，这是什么意思啊，
已经有5人回复
- “国家重大科学仪器设备开发专项”的英文翻译是啥？
已经有1人回复
- 请问这是什么仪器？
已经有13人回复
- 这到底是什么仪器？
已经有2人回复
- 大家来帮帮忙，急，看看这是什么仪器
已经有3人回复
- [有奖系列调查之一] 材料科学与工程研究中最常用的一类仪器设备是什么？
已经有235人回复
- 请教一下各位这个玻璃仪器叫什么？
已经有15人回复
- 【讨论】感慨一下为啥中国酒搞不出自己的电镜、ICPMS等高端仪器
已经有32人回复
- 【求助】水处理实验室一般有啥仪器设备
已经有10人回复
- 这两样仪器是干啥用的 名称是啥【有效期至2010年12月20日】
已经有8人回复
- 【求助】拉曼光谱特征峰降低了4个波数？是什么原因造成的呢
已经有15人回复
- 【求助】请大家帮我看下这个仪器叫啥名？谢谢
已经有30人回复
longage.gene
银虫 (小有名气)
- MicEPI: 1
- 应助: 4 (幼儿园)
- 金币: 743.7
- 散金: 4
- 红花: 2
- 帖子: 121
- 在线: 296.9小时
- 虫号: 1359202
- 注册: 2011-08-02
- 专业: 微生物生理与生物化学
2楼2011-08-10 16:27:23
jayzhao520
木虫 (正式写手)
- 应助: 10 (幼儿园)
- 金币: 1985
- 散金: 231
- 红花: 2
- 帖子: 526
- 在线: 84.6小时
- 虫号: 1102926
- 注册: 2010-09-19
- 性别: GG
- 专业: 金属材料的力学行为
3楼2011-08-12 12:46:32
longage.gene
银虫 (小有名气)
- MicEPI: 1
- 应助: 4 (幼儿园)
- 金币: 743.7
- 散金: 4
- 红花: 2
- 帖子: 121
- 在线: 296.9小时
- 虫号: 1359202
- 注册: 2011-08-02
- 专业: 微生物生理与生物化学
4楼2011-08-13 22:57:30
ganchao1776
至尊木虫 (职业作家)
- 应助: 18 (小学生)
- 金币: 11638.1
- 散金: 1811
- 红花: 30
- 帖子: 3407
- 在线: 900.8小时
- 虫号: 330935
- 注册: 2007-03-24
- 性别: GG
- 专业: 生物大分子结构与功能
5楼2011-08-17 23:54:13
50