你好：
阅读你之前的帖子，感觉你在微生物方面很专业，所以想请教个问题。我向NCBI提交序列后，也收到了一封关于样品信息的回信，由于我第一次弄不太懂，能否麻烦你帮忙看一下吗！谢谢！以下是genbank的回信：
[1] Is this sequence from:
a) a purified culture that contains only one microbial species or a vouchered specimen
b) enrichment culture: use of selective culture media to enrich for a set
of microorganisms with a particular phenotypic property, resulting in
a partially purified, mixed culture. Do not choose this option
for a purified strain or vouchered specimen.
c) bulk environmental DNA (no cultures or other vouchers available):
PCR-amplified directly from source/host DNA.
If this is from bulk environmental DNA, was it amplified using:
i) universal primers - amplifies DNA from a broad range of organisms
or
ii) species-specific primers - amplifies DNA from a single species
[2] Provide a clone/isolate/strain/specimen voucher or other unique identifier
for your record, using accepted biorepository acronyms where possible.
[3] Provide additional details describing the environmental conditions and
geographic location where this sequence or organism was isolated.
回复此楼
送红花一朵