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feiye2214

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[求助] 向genbank提交序列之后，收到一信件，需要提供有关样品的信息？有人遇到过么？救赎

小弟前天向genbank提交了一个cDNA全序列，今天收到回信，需要再提供一些信息，我第一次提交序列，各位大侠帮忙看看，到底是要什么东西？
难道是：样品采集的地点，采集方式？获得cDNA的方式和方法？跑胶照片？
求救赎！下面是信件的内容，照片也是
Dear GenBank Submitter:

Please find your assigned Accession Number(s) in the letter below.

Please provide a strain, isolate, clone, specimen voucher, OR an
alpha-numeric code used in your laboratory to distinguish your
submission(s) from others in the database. Note we are requesting
that you provide only one of these identifiers, if available.

For more information, please see:
http://www.ncbi.nlm.nih.gov/books/NBK53701/

Send this information to: gb-admin@ncbi.nlm.nih.gov

Thank you and we look forward to hearing from you soon.

Sincerely,

The GenBank Submissions Staff
Bethesda, Maryland USA

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1楼 2011-08-10 11:14:54

已阅回复此楼关注TA 给TA发消息送TA红花 TA的回帖

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引用回帖:

2楼: Originally posted by zemin_fang at 2011-08-10 11:23:13
主要是提供你递交的序列原始来源菌株的名称，如果是克隆，给你的克隆起个名字，以保证你的序列名称在genbank'中是唯一的。

你好：
   阅读你之前的帖子，感觉你在微生物方面很专业，所以想请教个问题。我向NCBI提交序列后，也收到了一封关于样品信息的回信，由于我第一次弄不太懂，能否麻烦你帮忙看一下吗！谢谢！以下是genbank的回信：
[1] Is this sequence from:

  a) a purified culture that contains only one microbial species or a vouchered specimen

  b) enrichment culture: use of selective culture media to enrich for a set
   of microorganisms with a particular phenotypic property, resulting in
   a partially purified, mixed culture. Do not choose this option
   for a purified strain or vouchered specimen.

  c) bulk environmental DNA (no cultures or other vouchers available):
   PCR-amplified directly from source/host DNA.

If this is from bulk environmental DNA, was it amplified using:
      i) universal primers - amplifies DNA from a broad range of organisms
      or
      ii) species-specific primers - amplifies DNA from a single species

[2] Provide a clone/isolate/strain/specimen voucher or other unique identifier
for your record, using accepted biorepository acronyms where possible.

[3] Provide additional details describing the environmental conditions and
geographic location where this sequence or organism was isolated.

Send your response to:  gb-admin@ncbi.nlm.nih.gov

If we do not hear from you by Jul  1, 2015, all of your submission(s) will
be deleted from the processing queue.

For your reference, please find your preliminary flatfile below
with the information we currently have.