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夏幼男最新德国应化：MTT细胞成像
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a,b) Confocal microscopy images and c,d) photoacoustic microscopy (PAM) images of fibroblasts cultured on cover glasses. The cells were incubated in the a,c) presence and b,d) absence of MTT, respectively, for 3 h, followed by fixation and staining for f-actin with Alexa 568-phalloidin. e,f) Superimposed images, indicating that essentially all the cells were stained by MTT formazan. g) PAM images of fibroblasts cultured on cover glasses in the presence of MTT at the indicated concentration and duration of time. The photoacoustic (PA) signal amplitude increased as the concentration of MTT and the incubation time were increased. h) The amount of MTT formazan (picogram, pg) and PA signal intensity per cell as a function of MTT concentration and incubation time. After PAM imaging, the MTT formazan in each sample was extracted by dissolution with 1-propanol and then quantified using a spectrophotometer. The MTT formazan concentrations were calculated from the calibration curve (see Figure S1 in the Supporting Information) and then divided by the cell numbers (n=3). The PA signal intensity per cell was calculated from the PA image and cell number (n=4). Data are presented as mean value standard deviation (SP=spectrophotometry).  |
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