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【答案】应助回帖
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amisking(金币+5, EPI+1): 鼓励应助 2011-06-24 20:15:28
枚馨言子: 回帖置顶 2012-02-21 21:07:57
区别是基因型不一样，也可以提取质粒，参考下面：
大肠杆菌DH5α：
F-endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169,hsdR17(rK- mK+), λ–
An Hoffman-Berling 1100 strain derivative (Meselson68)
Promega also lists phoA
nalidixic acid resistant
References:
FOCUS (1986) 8:2, 9.
Hanahan, D. (1985) in DNA Cloning: A Practical Approach (Glover, D.M., ed.), Vol. 1, p. 109,IRL Press, McLean, Virginia.
Grant, S.G.N. et al. (1990) Proc. Natl. Acad. Sci. USA 87: 4645-4649 PMID 2162051.
Meselson M. and Yuan R. (1968) Nature 217:1110 PMID 4868368.
大肠杆菌S17-1 Genotype: recA pro hsdR RP4-2-Tc::Mu-Km::Tn7
Growth Conditions: Protocol: plus trimethopirm (10 mcg/ml)
Temperature: 37.0°C
Applications: transformation host
Comments: Recommended mobilization host for pARO180, pARO190, pARO181, and pARO191 (ATCC 77123-77126, respectively) because it contains chromosomally integrated tra genes. [29601]
A kanamycin-sensitive strain devoid of the E. coli K-12-specific restriction system allowing efficient uptake of foreign DNA. [41566]
The properties of streptomycin, trimethoprim, and spectinomycin resistance were verified.
RP4-Tc::Mu-Km::Tn7 is integrated into the chromosome. [29601]
References: 29601: Parke D. Construction of mobilizable vectors derived from plasmids RP4, pUC18 and pUC19. Gene 93: 135-137, 1990. PubMed: 2227423
30953: Simon R, et al. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria. Bio-Technology 1: 784-791, 1983.
41566: McFarlane GJ, et al. A simplified method for conjugal gene transfer into the filamentous cyanobacterium Anabaena sp. ATCC 27893. J. Microbiol. Methods 6: 301-305, 1987. |
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