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【答案】应助回帖
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sltmac(金币+1): 感谢应助~~ 2011-06-24 18:00:00
hmily601(金币+12, 翻译EPI+1): 2011-06-24 18:09:05
Synthesis-enabled functional group deletions reveal key underpinnings of amphotericin B ion channel and antifungal activities合成激活官能团缺失显示两性霉素B离子通道和抗真菌活性的关键基础
Voltage Clamp Single Channel Recordings.电压钳单通道记录 Experiments were performed using a Warner Instruments planar lipid bilayer workstation. 试验在Warner Instruments 平面脂双层工作站上进行。Similar to prior studies (1), planar lipid membranes were formed from PC:phosphatidylethanolamine:ergosterol 40∶20∶1 over a 100–150 μm hole in a Teflon® sheet. 与先前的研究相似，平面脂质膜由PC：磷脂酰乙醇胺：麦角固醇40:20:1透过特氟龙薄片中的100-150μm 的孔形成。The bathing solution was 2.5 M KCl 10 mM potassium phosphate (pH 7.0). bathing液为2.5 M KCl 10 mM磷酸钾 (pH 7.0) 。Small molecules in DMSO were added, and the resulting solutions were stirred for 10 min with zero applied potential. 加入溶解在二甲基亚砜中的小分子，得到的溶液在零电压下搅拌十分钟。Then, 150 mV of potential was applied across the membrane and channel formation was monitored.随后，使用150mV的跨膜电位，监测通道的形成。 |
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