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dfbb2006金虫 (小有名气)
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[求助]
在线坐等论文摘要，求高手帮助改正！有汉语对应的英语翻译！老师说有些问题！~
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我的导师看过之后说英语有些问题，但是本人才疏学浅，实在不会了，求高手帮忙调整一下顺序，或者那个词、词组用的不对帮忙更换一下，不胜感激！本人新手注册时间尚短，奉献全额金币，望答复！~ 摘 要 本文通过活性筛选，选用人肝癌Hep G2 细胞株用他克林诱导细胞毒性，将乌苏里瓦韦（Lepisorus ussuriensis）各溶剂浸膏作用于经他克林处理过的Hep G2细胞，结果显示乌苏里瓦韦乙酸乙酯浸膏能够有效的降低他克林对Hep G2细胞产生的毒性，具有较好的细胞保护作用。 乌苏里瓦韦乙酸乙酯浸膏能够减少脂多糖（LPS）/D-氨基半乳糖（D-GalN）引起的急性肝损伤小鼠的死亡率。能显著降低由LPS/D-GalN引起的肝损伤小鼠的谷丙转氨酶（ALT）和谷草转氨酶（AST）活性，降低丙二醛（MDA）含量，升高超氧化物歧化酶（SOD）活性。结果显示乌苏里瓦韦乙酸乙酯浸膏通过抗氧化活性对LPS/D-GalN所致的急性肝损伤小鼠具有保护作用。 综合应用多种层析分离技术，从乌苏里瓦韦中分离得到6个化合物，并进一步通过波谱分析（1H-NMR，13C-NMR等）鉴定了6个化合物的结构分别是：2-丙烯酸-3-（4-羟基苯基）-乙酯（1），4-乙氧基苯甲酸（2），圣草酚-7-O-葡萄糖苷（3），4’,5,7-三羟基-3-甲氧基黄酮-7-O-β-D-葡萄糖苷（4），3’,4,5,7-四羟基-3-甲氧基黄酮-7-O-β-D-葡萄糖苷（5），2-（3,4-二羟基苯基）乙基-6-[3-（3,4-二羟基苯基）-2 -丙烯酸]- β-D-葡萄糖苷（6）。 其中化合物1-6均能有效的降低他克林对Hep G2细胞产生的毒性，具有较好的细胞保护作用。 目前对乌苏里瓦韦肝保护活性成分的研究仍然较少。因此，分离乌苏里瓦韦肝保护活性成分，并进一步确定其肝保护活性成分的结构有着重要的理论和实际意义。 关键词：瓦韦；动物实验；结构鉴定；细胞实验 Abstract In vitro screen assay, use of human hepatoma Hep G2 cell line induced by tacrine cytotoxicity, the L.ussuriensis role in the solvent extracts of tacrine treated by Hep G2 cells, The results showed that ethyl acetate extract of L.ussuriensis can effectively reduce tacrine on the toxicity of Hep G2 cells. Ethyl acetate extract of Lepisorus ussuriensis bring down the activity of AST and ALT in the LPS and D-GalN induced hepatic serum, bring up the liver SOD activity, reduced the content of MDA, and improve the survival rate of mice, which has significan protection to hepatic mice. Experimental study on L.ussuriensis the the chemical composition, using various separation methods, 6 compounds were isolated from the L.ussuriensis and further analyzed by spectral Identified the structure of 6 compounds were : 2-propenoic acid-3-(4-hydroxyphenyl)-ethyl ester(1), 4-ethoxybenzoic acid(2), eriodictyol-7-O-glucoside(3), 4’,5,7-trihydroxy-3-methoxy flavone-7-O-β-D- glucose -ide(4), 3’,4’,5,7-tetrahydroxy -3-methoxy flavone-7-O-β-D- glucoside(5), 2-(3,4-dihy-droxyphenyl)ethyl-6-[3-(3,4-dihydroxyphenyl)-2-propenoate]-β-D-glucoside(6). Compounds 1-6 isolated from the L.ussuriensis can effectively reduce toxicity of Hep G2 cells. Current research on Liver protection active ingredient of L.ussuriensis is still not popular. Therefore, it is of important theoretical and practical significance on the separation of Liver protection active ingredient of L.ussuriensis and further identify the structure of Liver protection active ingredient. Key words: Lepisorus ussuriensis; Chemical composition; Animal experiments; Structure identification https://good.gd/1259131.htm这可以下载！~ [ Last edited by dfbb2006 on 2011-6-8 at 00:43 ] |
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zuodg
铁杆木虫 (正式写手)
有为青年
- 翻译EPI: 180
- 应助: 5 (幼儿园)
- 贵宾: 0.015
- 金币: 6765.2
- 散金: 48
- 红花: 18
- 帖子: 500
- 在线: 285.5小时
- 虫号: 1055187
- 注册: 2010-07-10
- 性别: GG
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2楼2011-06-08 11:38:22
zuodg
铁杆木虫 (正式写手)
有为青年
- 翻译EPI: 180
- 应助: 5 (幼儿园)
- 贵宾: 0.015
- 金币: 6765.2
- 散金: 48
- 红花: 18
- 帖子: 500
- 在线: 285.5小时
- 虫号: 1055187
- 注册: 2010-07-10
- 性别: GG
- 专业: 外国语言
【答案】应助回帖
dfbb2006(金币+15, 翻译EPI+1): 十分感谢，看来的我错误还真是多！~~~ 2011-06-08 19:36:40
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Abstract In vitro bioactivity assay, cytotoxicity was induced by tacrine with human hepatoma cell line Hep G2. Extracts of Lepisorus ussuriensis with various organic solvents were applicated in treatment of Hep G2, which was treated as above-mentioned. Results indicated that ethyl acetate extract effectively reduced cytotoxicity of Hep G2 caused by tacrine and thus played a role in cell protection. Ethyl acetate extract of Lepisorus ussuriensis lowers death rate of acute hepatic injury mice caused by decrease of LPS/ D-GalN, lowers significantly activities of ALT and AST of hepatic injury mice caused by LPS/D-GalN and reduces content of MDA, as well as bring up activities of SOD. Results indicate through activities of antioxidant ethyl acetate extract of Lepisorus ussuriensis play a role in cell protection of acute liver injury mice caused by LPS/D-GalN. Various isolation techniques were applicated. Six compounds were isolated from Lepisorus ussuriensis, which were characterized through 1H-NMR to be 2-propenoic acid-3-(4-hydroxyphenyl)-ethyl ester(1), 4-ethoxybenzoic acid(2), eriodictyol-7-O-glucoside(3), 4’,5,7-trihydroxy-3-methoxy flavone-7-O-β-D- glucose-ide(4), 3’,4’,5,7-tetrahydroxy -3-methoxy flavone-7-O-β-D- glucoside(5), 2-(3,4-dihy-droxyphenyl)ethyl-6-[3-(3,4-dihydroxyphenyl)-2-propenoate]-β-D-glucoside(6). Among them, all compounds can effectively reduce cytotoxicity of Hep G2 caused by tacrine and thus play a role in protection of cells. Currently, relatively less is reported on Liver protection active ingredients. Therefore, it is of theoretical and practical value to further isolate and characterize liver protection active ingredients of L.ussuriensis. Key words: Lepisorus ussuriensis; Chemical composition; Animal experiments; Structure identification |
3楼2011-06-08 14:10:15