24小时热门版块排行榜    

查看: 1814  |  回复: 7
本帖产生 1 个 MicEPI ,点击这里进行查看
当前只显示满足指定条件的回帖,点击这里查看本话题的所有回帖

mirror3895

铁杆木虫 (正式写手)

[求助] NCBI登录号申请相关问题

分离的菌株想在NCBI上申请序列号,用sequin提交相关资料后,对方给我发回这么一封信:
Dear Dr. Huang:

We are currently processing your GenBank records JF772465-JF772548
and have come across a point that requires your attention.

BLAST similarity search analysis indicates that the following sequence
may be misassembled:

JF772520

This sequence contains a mixture of plus and minus strand sequence which
is biologically incorrect. We have appended an example BLAST result below
for your information.  Please provide a revised FASTA file with the
corrected sequence.

In addition, BLAST similarity search analysis indicates that many
of your sequences contain little or no similarity to ribosomal RNA
sequences in the database at the 5' and 3' ends.  Ribosomal RNAs are
generally highly conserved and thus, we would expect to see similarity
to other rRNA sequences over the entire length.

这是信的内容。以前也申请过,但是没遇到过这个问题,看不太懂。不知道哪位高手能帮忙解答一下吗?先谢谢了
回复此楼

» 猜你喜欢

» 本主题相关价值贴推荐,对您同样有帮助:

已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

mirror3895

铁杆木虫 (正式写手)

引用回帖:
Originally posted by scelab at 2011-05-23 18:42:48:
This sequence contains a mixture of plus and minus strand sequence which
is biologically incorrect. We have appended an example BLAST result below
for your information.  Please provide a revise ...

再问一下,我的序列是测序公司拼接好后直接返给我的,难道要一个个去检查吗?
3楼2011-05-23 19:08:13
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
查看全部 8 个回答

scelab

荣誉版主 (文坛精英)

小木虫之有关部门负责人

【答案】应助回帖

mirror3895(金币+2): 谢谢解答呵呵 2011-05-23 19:07:14
amisking(EPI+1): 鼓励应助 2011-05-23 20:22:36
mirror3895(金币+1): 2011-05-30 13:54:20
This sequence contains a mixture of plus and minus strand sequence which
is biologically incorrect. We have appended an example BLAST result below
for your information.  Please provide a revised FASTA file with the
corrected sequence.
这个说你正负链有拼错的地方,根据他推荐的seq,看看自己到底哪里错了(如果确定没问题,可以回复说就是这样)

In addition, BLAST similarity search analysis indicates that many
of your sequences contain little or no similarity to ribosomal RNA
sequences in the database at the 5' and 3' ends.  Ribosomal RNAs are
generally highly conserved and thus, we would expect to see similarity
to other rRNA sequences over the entire length.
你的3,5端的序列是测出来的吗?还是引物加上去的?因为这部分的相当保守,而admin认为你的seq理由非正常的序列。
你的seq是全长吗?
他问到这些问题,之前可能就混过去了,现在不行了,呵呵,大家都很牛的~

ps:对admin的专业及敬业表示钦佩~~~
小木虫之有关部门负责人
2楼2011-05-23 18:42:48
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

scelab

荣誉版主 (文坛精英)

小木虫之有关部门负责人

【答案】应助回帖

mirror3895(金币+1): 2011-05-24 18:51:05
引用回帖:
Originally posted by mirror3895 at 2011-05-23 19:08:13:
再问一下,我的序列是测序公司拼接好后直接返给我的,难道要一个个去检查吗?

最好,不能全信公司。。。
而且,你的序列有载体序列吗?要去掉哦~
小木虫之有关部门负责人
4楼2011-05-23 19:33:42
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

mirror3895

铁杆木虫 (正式写手)

引用回帖:
Originally posted by scelab at 2011-05-23 19:33:42:
最好,不能全信公司。。。
而且,你的序列有载体序列吗?要去掉哦~

确定载体都去掉了,因为是用sequin提交的,可以检测载体污染
5楼2011-05-23 19:57:37
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
最具人气热帖推荐 [查看全部] 作者 回/看 最后发表
[考研] 271材料工程求调剂 +7 .6lL 2026-03-18 7/350 2026-03-20 09:10 by xingguangj
[考研] 329求调剂 +6 想上学吖吖 2026-03-19 6/300 2026-03-20 09:00 by 每天只摆一小会
[考研] 材料学硕318求调剂 +5 February_Feb 2026-03-19 5/250 2026-03-19 23:51 by 23Postgrad
[考研] 0703化学调剂 ,六级已过,有科研经历 +12 曦熙兮 2026-03-15 12/600 2026-03-19 19:42 by maocaozhuxi
[考研] 本人考085602 化学工程 专硕 +17 不知道叫什么! 2026-03-15 19/950 2026-03-19 15:06 by 尽舜尧1
[考研] 0703化学调剂 +5 pupcoco 2026-03-17 8/400 2026-03-19 13:58 by houyaoxu
[考研] 一志愿福大288有机化学,求调剂 +3 小木虫200408204 2026-03-18 3/150 2026-03-19 13:31 by houyaoxu
[考研] 346求调剂[0856] +3 WayneLim327 2026-03-16 6/300 2026-03-19 11:21 by WayneLim327
[考研] 一志愿华中科技大学,080502,354分求调剂 +4 守候夕阳CF 2026-03-18 4/200 2026-03-18 22:16 by li123456789.
[考研] 311求调剂 +6 26研0 2026-03-15 6/300 2026-03-18 14:43 by haxia
[考研] 331求调剂(0703有机化学 +7 ZY-05 2026-03-13 8/400 2026-03-18 14:13 by 007_lilei
[考研] 303求调剂 +4 睿08 2026-03-17 6/300 2026-03-18 11:01 by Iveryant
[考研] 301求调剂 +9 yy要上岸呀 2026-03-17 9/450 2026-03-18 08:58 by 无际的草原
[考研] 有没有道铁/土木的想调剂南林,给自己招师弟中~ +3 TqlXswl 2026-03-16 7/350 2026-03-17 15:23 by TqlXswl
[考研] 材料与化工专硕调剂 +5 heming3743 2026-03-16 5/250 2026-03-17 14:03 by 勇敢太监王公公
[考研] 11408 一志愿西电,277分求调剂 +3 zhouzhen654 2026-03-16 3/150 2026-03-17 07:03 by laoshidan
[基金申请] 今年的国基金是打分制吗? 50+3 zhanghaozhu 2026-03-14 3/150 2026-03-16 17:07 by 北京莱茵润色
[考研] 304求调剂 +3 曼殊2266 2026-03-14 3/150 2026-03-16 16:39 by houyaoxu
[考研] 326求调剂 +3 mlpqaz03 2026-03-15 3/150 2026-03-16 07:33 by Iveryant
[考研] 266求调剂 +4 学员97LZgn 2026-03-13 4/200 2026-03-14 08:37 by zhukairuo
信息提示
请填处理意见