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mirror3895

铁杆木虫 (正式写手)

[求助] NCBI登录号申请相关问题

分离的菌株想在NCBI上申请序列号,用sequin提交相关资料后,对方给我发回这么一封信:
Dear Dr. Huang:

We are currently processing your GenBank records JF772465-JF772548
and have come across a point that requires your attention.

BLAST similarity search analysis indicates that the following sequence
may be misassembled:

JF772520

This sequence contains a mixture of plus and minus strand sequence which
is biologically incorrect. We have appended an example BLAST result below
for your information.  Please provide a revised FASTA file with the
corrected sequence.

In addition, BLAST similarity search analysis indicates that many
of your sequences contain little or no similarity to ribosomal RNA
sequences in the database at the 5' and 3' ends.  Ribosomal RNAs are
generally highly conserved and thus, we would expect to see similarity
to other rRNA sequences over the entire length.

这是信的内容。以前也申请过,但是没遇到过这个问题,看不太懂。不知道哪位高手能帮忙解答一下吗?先谢谢了
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scelab

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小木虫之有关部门负责人

【答案】应助回帖

mirror3895(金币+2): 谢谢解答呵呵 2011-05-23 19:07:14
amisking(EPI+1): 鼓励应助 2011-05-23 20:22:36
mirror3895(金币+1): 2011-05-30 13:54:20
This sequence contains a mixture of plus and minus strand sequence which
is biologically incorrect. We have appended an example BLAST result below
for your information.  Please provide a revised FASTA file with the
corrected sequence.
这个说你正负链有拼错的地方,根据他推荐的seq,看看自己到底哪里错了(如果确定没问题,可以回复说就是这样)

In addition, BLAST similarity search analysis indicates that many
of your sequences contain little or no similarity to ribosomal RNA
sequences in the database at the 5' and 3' ends.  Ribosomal RNAs are
generally highly conserved and thus, we would expect to see similarity
to other rRNA sequences over the entire length.
你的3,5端的序列是测出来的吗?还是引物加上去的?因为这部分的相当保守,而admin认为你的seq理由非正常的序列。
你的seq是全长吗?
他问到这些问题,之前可能就混过去了,现在不行了,呵呵,大家都很牛的~

ps:对admin的专业及敬业表示钦佩~~~
小木虫之有关部门负责人
2楼2011-05-23 18:42:48
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mirror3895

铁杆木虫 (正式写手)

引用回帖:
Originally posted by scelab at 2011-05-23 18:42:48:
This sequence contains a mixture of plus and minus strand sequence which
is biologically incorrect. We have appended an example BLAST result below
for your information.  Please provide a revise ...

再问一下,我的序列是测序公司拼接好后直接返给我的,难道要一个个去检查吗?
3楼2011-05-23 19:08:13
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scelab

荣誉版主 (文坛精英)

小木虫之有关部门负责人

【答案】应助回帖

mirror3895(金币+1): 2011-05-24 18:51:05
引用回帖:
Originally posted by mirror3895 at 2011-05-23 19:08:13:
再问一下,我的序列是测序公司拼接好后直接返给我的,难道要一个个去检查吗?

最好,不能全信公司。。。
而且,你的序列有载体序列吗?要去掉哦~
小木虫之有关部门负责人
4楼2011-05-23 19:33:42
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mirror3895

铁杆木虫 (正式写手)

引用回帖:
Originally posted by scelab at 2011-05-23 19:33:42:
最好,不能全信公司。。。
而且,你的序列有载体序列吗?要去掉哦~

确定载体都去掉了,因为是用sequin提交的,可以检测载体污染
5楼2011-05-23 19:57:37
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mirror3895

铁杆木虫 (正式写手)

引用回帖:
Originally posted by mirror3895 at 2011-05-23 19:57:37:
确定载体都去掉了,因为是用sequin提交的,可以检测载体污染

感觉你是高手啊,请教一下,我现在该怎么做呢?
6楼2011-05-23 19:59:25
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scelab

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小木虫之有关部门负责人

【答案】应助回帖

mirror3895(金币+1): 2011-05-23 22:01:33
引用回帖:
Originally posted by mirror3895 at 2011-05-23 19:59:25:
感觉你是高手啊,请教一下,我现在该怎么做呢?

不高~
JF772465-JF772548。。。你的是批量生产啊。。。
不明真相,没法提供意见~
建议自己把每条seq都比对分析下~~~(这样最好)
但是admin只指出一条来,那就重点搞这一条吧,请教下身边的高手,看看到底问题在哪里?(个人感觉公司拼错的可能性大)
祝好运~
小木虫之有关部门负责人
7楼2011-05-23 21:03:01
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mirror3895

铁杆木虫 (正式写手)

引用回帖:
Originally posted by scelab at 2011-05-23 21:03:01:
不高~
JF772465-JF772548。。。你的是批量生产啊。。。
不明真相,没法提供意见~
建议自己把每条seq都比对分析下~~~(这样最好)
但是admin只指出一条来,那就重点搞这一条吧,请教下身边的高手,看看到 ...

因为测了很多嘛,所以一起申请的。我们每次测序结果都是别人帮拼接好的,哎
8楼2011-05-23 22:02:33
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