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zuodg

铁杆木虫 (正式写手)

有为青年

翻译EPI: 180
应助: 5 (幼儿园)
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金币: 6765.2
散金: 48
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帖子: 500
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虫号: 1055187
注册: 2010-07-10
性别: GG
专业: 外国语言

【答案】应助回帖

★
sltmac(金币+1): 谢谢交流~~ 2011-04-28 10:43:01
dong5705(金币+48, 翻译EPI+1): 很不错了比我写的好多了呵呵非常感谢 2011-04-28 10:56:45

引用回帖:

Originally posted by dong5705 at 2011-04-27 20:03:26:
提要

目的：进一步完善大肠杆菌和金黄色葡萄球菌细菌生物膜模型，建立完善的细菌生物膜模型方法；考察具有抗菌作用的中药单体及人参总皂苷对细菌生物膜的作用；筛选出对细菌生物膜具有较强抑制作用的人参皂苷 ...

Abstract
Purpose: Improve bacterial biofilm model of E.coli and Staphylococcus aureus, and establish protocol for excellent bacterial biofilm model. Investigate effect of antibacterial Chinese medicine monomer and ginsenoside on bacterial biofilm. Screen ginsenoside monomer which inhibits bacterial biofilm. Observe combined affect of ginsenoside and Chinese medicine monomer on bacterial biofilm.
Method: Observe formation and morphology of bacterial biofilm with SEM and LSCM. With E.coli and Staphylococcus aureus as screening model of bacterial biofilm, coculture of bacterial biofilm with Chinese medicine monomers, ginsenosides and single ginsenoside, and measure growth capacity of bacteria and biofilm based on OD values from microplate reader. Observe inhibitory capacity of different samples on bacteria and biofilms through inhibition rate. Screen other Chinese medicine monomers which have combined effect on bacterial biofilm with ginsenoside by trace broth dilution method.
Result: With the application of SEM and LSCM, it is observed that biofilm of E.coli and Staphylococcus aureus begin to generate after 24 hrs’ culture. Ginsenosides have strong inhibitory effect on E.coliu and its biofilm, while they only inhibit Staphylococcus aureus and its biofilm to some extent. RB1、Rb2、Rb3、Rc、Rd, which inhibit E.coliu and its biofilm greatly, were obtained after screening of ginsenoside monomers
Combined medication shows, combination of RB2 and Ferulic acid have collaborative inhibition on E.coliu and its biofilm
Conclusion: With application of SEM and CLSM, bacterial biofilm model is further improved. Bacterial biofilm model as screening model of chronic infection drugs is furthrt confirmed through investigation of impact of Chinese medicine monomers on bacterial and BBF. Ginsensides which inhibit bacterial and BBF greatly were obtained. In addition, ginsenoside which have collaborative effect with Ferulic acid was also obtained, which lays good foundation for further combined medicate with ginseng and treatment of chronic infection disease.
Key words: E.coli Staphylococcus aureus biofilm ginsenoside

今晚不在状态，再加上对生物膜不太熟悉，所以翻译的不好请见谅啊

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