Abstract
Purpose: Improve bacterial biofilm model of E.coli and Staphylococcus aureus, and establish protocol for excellent bacterial biofilm model. Investigate effect of antibacterial Chinese medicine monomer and ginsenoside on bacterial biofilm. Screen ginsenoside monomer which inhibits bacterial biofilm. Observe combined affect of ginsenoside and Chinese medicine monomer on bacterial biofilm.
Method: Observe formation and morphology of bacterial biofilm with SEM and LSCM. With E.coli and Staphylococcus aureus as screening model of bacterial biofilm, coculture of bacterial biofilm with Chinese medicine monomers, ginsenosides and single ginsenoside, and measure growth capacity of bacteria and biofilm based on OD values from microplate reader. Observe inhibitory capacity of different samples on bacteria and biofilms through inhibition rate. Screen other Chinese medicine monomers which have combined effect on bacterial biofilm with ginsenoside by trace broth dilution method.
Result: With the application of SEM and LSCM, it is observed that biofilm of E.coli and Staphylococcus aureus begin to generate after 24 hrs’ culture. Ginsenosides have strong inhibitory effect on E.coliu and its biofilm, while they only inhibit Staphylococcus aureus and its biofilm to some extent. RB1、Rb2、Rb3、Rc、Rd, which inhibit E.coliu and its biofilm greatly, were obtained after screening of ginsenoside monomers
Combined medication shows, combination of RB2 and Ferulic acid have collaborative inhibition on E.coliu and its biofilm
Conclusion: With application of SEM and CLSM, bacterial biofilm model is further improved. Bacterial biofilm model as screening model of chronic infection drugs is furthrt confirmed through investigation of impact of Chinese medicine monomers on bacterial and BBF. Ginsensides which inhibit bacterial and BBF greatly were obtained. In addition, ginsenoside which have collaborative effect with Ferulic acid was also obtained, which lays good foundation for further combined medicate with ginseng and treatment of chronic infection disease.
Key words: E.coli Staphylococcus aureus biofilm ginsenoside
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