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dong5705

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[求助] 高分求助微生物类细菌生物膜硕士研究生论文摘要汉译英急

提要

目的：进一步完善大肠杆菌和金黄色葡萄球菌细菌生物膜模型，建立完善的细菌生物膜模型方法；考察具有抗菌作用的中药单体及人参总皂苷对细菌生物膜的作用；筛选出对细菌生物膜具有较强抑制作用的人参皂苷单体；并考察其与中药单体联合应用对细菌生物膜的影响。
方法：利用扫描电子显微镜及激光共聚焦显微镜观察细菌生物膜的形成及形态；以大肠杆菌及金黄色葡萄球菌生物膜为筛选模型，将中药单体物质、人参总皂苷、各人参皂苷等物质与细菌及细菌生物膜共同培养，通过酶标仪读取的OD值来测定细菌及生物膜的生长能力，通过计算抑菌率来观察各样品对细菌及生物膜的抑制作用的大小；采用微量肉汤稀释法筛选与人参皂苷单体联合用药对细菌及生物膜产生影响的其他中药单体。
结果：通过扫描电子显微镜（SEM）及激光共聚焦扫描电子显微镜（CLSM）镜下观察的结果，确定大肠杆菌和金黄色葡萄球菌在培养24h时，形成生物膜；人参总皂苷对大肠杆菌及生物膜具有很强的抑制作用，对金黄色葡萄球菌及BBF有一定的抑制作用，但抑制作用较弱；筛选人参单体皂苷，得到Rb1、Rb2、Rb3、Rc、Rd及对大肠杆菌及细菌生物膜有很强的抑制作用；联合用药结果显示：Rb2与阿魏酸联合应用，对大肠杆菌BBF产生一定协同抑制作用。
结论：通过SEM和CLSM实验，进一步完善了细菌生物膜模型；通过中药单体对细菌及生物膜的作用，进一步验证了细菌生物膜模型作为筛选慢性感染性药物模型的可行性。筛选出对细菌生物膜具有较强抑制作用的人参皂苷单体；也筛选得到阿魏酸与人参皂苷单体联合应用对BBF产生协同抑制作用，这为下一步人参及人参与其它药物联合应用，治疗慢性感染性疾病的深入开发研究，打下良好的基础，并提供科学的依据。

关键词：大肠杆菌金黄色葡萄球菌生物膜人参皂苷

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好好学习，天天向上

1楼 2011-04-27 20:03:26

已阅回复此楼关注TA 给TA发消息送TA红花 TA的回帖

zuodg

铁杆木虫 (正式写手)

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【答案】应助回帖

★
sltmac(金币+1): 谢谢交流~~ 2011-04-28 10:43:01
dong5705(金币+48, 翻译EPI+1): 很不错了比我写的好多了呵呵非常感谢 2011-04-28 10:56:45

引用回帖:

Originally posted by dong5705 at 2011-04-27 20:03:26:
提要

目的：进一步完善大肠杆菌和金黄色葡萄球菌细菌生物膜模型，建立完善的细菌生物膜模型方法；考察具有抗菌作用的中药单体及人参总皂苷对细菌生物膜的作用；筛选出对细菌生物膜具有较强抑制作用的人参皂苷 ...

Abstract
Purpose: Improve bacterial biofilm model of E.coli and Staphylococcus aureus, and establish protocol for excellent bacterial biofilm model. Investigate effect of antibacterial Chinese medicine monomer and ginsenoside on bacterial biofilm. Screen ginsenoside monomer which inhibits bacterial biofilm. Observe combined affect of ginsenoside and Chinese medicine monomer on bacterial biofilm.
Method: Observe formation and morphology of bacterial biofilm with SEM and LSCM. With E.coli and Staphylococcus aureus as screening model of bacterial biofilm, coculture of bacterial biofilm with Chinese medicine monomers, ginsenosides and single ginsenoside, and measure growth capacity of bacteria and biofilm based on OD values from microplate reader. Observe inhibitory capacity of different samples on bacteria and biofilms through inhibition rate. Screen other Chinese medicine monomers which have combined effect on bacterial biofilm with ginsenoside by trace broth dilution method.
Result: With the application of SEM and LSCM, it is observed that biofilm of E.coli and Staphylococcus aureus begin to generate after 24 hrs’ culture. Ginsenosides have strong inhibitory effect on E.coliu and its biofilm, while they only inhibit Staphylococcus aureus and its biofilm to some extent. RB1、Rb2、Rb3、Rc、Rd, which inhibit E.coliu and its biofilm greatly, were obtained after screening of ginsenoside monomers
Combined medication shows, combination of RB2 and Ferulic acid have collaborative inhibition on E.coliu and its biofilm
Conclusion: With application of SEM and CLSM, bacterial biofilm model is further improved. Bacterial biofilm model as screening model of chronic infection drugs is furthrt confirmed through investigation of impact of Chinese medicine monomers on bacterial and BBF. Ginsensides which inhibit bacterial and BBF greatly were obtained. In addition, ginsenoside which have collaborative effect with Ferulic acid was also obtained, which lays good foundation for further combined medicate with ginseng and treatment of chronic infection disease.
Key words: E.coli Staphylococcus aureus biofilm ginsenoside

今晚不在状态，再加上对生物膜不太熟悉，所以翻译的不好请见谅啊