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wwchen072(½ð±Ò+10): 2011-04-26 10:42:37
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ÊÇEIÊÕ¼£¬ÊÕ¼ÐÅÏ¢ÈçÏÂËùʾ£º Accession number: 20104813436451 Title: Surfaced-enhanced Raman spectroscopic study on single living human nasopharyngeal carcinoma cells incubated with colloidal gold Authors: Huang, Hao1 ; Pan, Jian-Ji3 ; Chen, Wei-Wei1, 2 ; Chen, Qi-Song3 ; Feng, Shang-Yuan2 ; Su, Ying3 ; Xu, Xiong-Wei4 ; Chen, Rong2 Author affiliation: 1 Department of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China 2 Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou 350007, China 3 Fujian Provincial Cancer Hospital, Fuzhou 350014, China 4 The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, China Corresponding author: Chen, R. (chenr@fjnu.edu.cn) Source title: Guang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy and Spectral Analysis Abbreviated source title: Guang Pu Xue Yu Guang Pu Fen Xi Volume: 30 Issue: 11 Issue date: November 2010 Publication year: 2010 Pages: 2981-2984 Language: Chinese ISSN: 10000593 CODEN: GYGFED Document type: Journal article (JA) Publisher: Science Press, 18,Shuangqing Street,Haidian, Beijing, 100085, China Abstract: The surface-enhanced Raman scattering (SERS) spectroscopy and normal Raman spectroscopy of single living human nasopharyngeal carcinoma cells(CNE-1) were tested and analyzed by gold nanoparticles incubation into cells. Six obvious Raman bands (718, 1001, 1123, 1336, 1446 and 1660 cm-1) were observed in the normal Raman spectroscopy of living CNE-1 cells. The characteristic Raman bands in the SERS spectra of living cells were tentatively assigned. Colloidal gold particles that were introduced inside cells result in strongly enhanced Raman signals of the native chemical constituents of the cells, and over twenty SERS Raman bands were observed in the SERS spectroscopy of living CNE-1 cells. The Raman lines of 1026, 1097, 1336 and 1 585 cm-1 were assigned to vibrations of the DNA backbone, which confirms that some gold nanoparticles were able to enter the nucleus. The results showed that, based on colloidal gold, the SERS spectroscopy might provide a sensitive and structurally selective detecting method for native chemicals inside a cell, such as DNA and phenylalanine. Number of references: 11 Main heading: Cells Controlled terms: Amino acids - Cell culture - Colloid chemistry - Colloids - Gold - Nanoparticles - Raman scattering - Raman spectroscopy - Scattering - Spectroscopic analysis - Surfaces Uncontrolled terms: Chemical constituents - Colloidal gold - Colloidal gold particles - Gold colloid - Gold Nanoparticles - Living cell - Nasopharyngeal carcinoma cells - Normal raman spectroscopies - Raman bands - Raman lines - Raman signal - Raman spectroscopic study - Selective detecting methods - SERS spectrum - Surface-enhanced Raman scattering - Surface-enhanced raman scattering spectroscopies Classification code: 951 Materials Science - 933 Solid State Physics - 931 Classical Physics; Quantum Theory; Relativity - 801 Chemistry - 761 Nanotechnology - 741.1 Light/Optics - 708 Electric and Magnetic Materials - 547.1 Precious Metals - 461 Bioengineering and Biology DOI: 10.3964/j.issn.1000-0593(2010)11-2981-04 Database: Compendex Compilation and indexing terms, ? 2011 Elsevier Inc. |
2Â¥2011-04-25 17:26:41
jiangfc
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3Â¥2011-04-25 17:34:24
jiangfc
Òø³æ (ÕýʽдÊÖ)
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¹§Ï²Â¥Ö÷£¬ÎÄÕÂҲͬʱ±»SCIÊÕ¼£¬¿ì°Ñ±Ò±ÒËÍÀ´¡£SCIÊÕ¼ºÅΪ000285284000024 £¬ÊÕ¼ÐÅÏ¢ÈçÏ£º FN ISI Export Format VR 1.0 PT J AU Huang, H Pan, JJ Chen, WW Chen, QS Feng, SY Su, Y Xu, XW Chen, R AF Huang Hao Pan Jian-ji Chen Wei Wei Chen Qi Song Feng Shang Yuan Su Ying Xu Xiong Wei Chen Rong TI Surfaced-Enhanced Raman Spectroscopic Study on Single Living Human Nasopharyngeal Carcinoma Cells Incubated with Colloidal Gold SO SPECTROSCOPY AND SPECTRAL ANALYSIS LA Chinese DT Article DE Surface-enhanced Raman scattering; Gold colloids; Human nasopharyngeal carcinoma cell line ID SCATTERING; NANOPARTICLES; MOLECULES AB The surface-enhanced Raman scattering (SERS) spectroscopy and normal Raman spectroscopy of single living human nasopharyngeal carcinoma cells(CNE 1) were tested and analyzed by gold nanoparticles incubation into cells Six obvious Raman bands (718 1 001 1 123 1 336 1 446 and 1 660 cm(-1))were observed in the normal Raman spectroscopy of living CNE 1 cells The characteristic Raman bands in the SERS spectra of living cells were tentatively assigned Colloidal gold particles that were introduced inside cells result in strongly enhanced Raman signals of the native chemical constituents of the cells and over twenty SERS Raman bands were observed in the SERS spectroscopy of living CNE-1 cells The Raman lines of 1 026 1 097 1 336 and 1 585 cm(-1) were assigned to vibrations of the DNA backbone which confirms that some gold nanoparticles were able to enter the nucleus The results showed that based on colloidal gold the SERS spectroscopy might provide a sensitive and structurally selective detecting method for native chemicals inside a cell such as DNA and phenylalanine C1 [Huang Hao; Chen Wei Wei] Fujian Univ Tradit Chinese Med, Dept Integrat Med, Fuzhou 350108, Peoples R China. [Chen Wei Wei; Feng Shang Yuan; Chen Rong] Fujian Normal Univ, Key Lab Optoelect Sci & Technol Med, Minist Educ, Fuzhou 350007, Peoples R China. [Pan Jian-ji; Chen Qi Song; Su Ying] Fujian Prov Canc Hosp, Fuzhou 350014, Peoples R China. [Xu Xiong Wei] Fujian Med Univ, Affiliated Hosp 2, Quanzhou 362000, Peoples R China. RP Huang, H, Fujian Univ Tradit Chinese Med, Dept Integrat Med, Fuzhou 350108, Peoples R China. CR BIN JQ, 2009, BIOSONSERS BIOELECTR, V25, P388 DULKEITH E, 2002, J PHYS REV LETT, V89, UNSP 203002 FENG SY, 2009, APPL SPECTROSC, V63, P1089 HANDLEY DA, 1989, COLLOIDAL GOLD KNEIPP K, 1998, APPL SPECTROSC, V52, P1493 KNEIPP K, 2002, APPL SPECTROSC, V56, P150 LO KW, 2004, CANCER CELL, V5, P423 NIE SM, 1997, SCIENCE, V275, P1102 SANDHU KK, 2002, BIOCONJUGATE CHEM, V13, P3 SMITH E, 2005, MODERN RAMAN SPECTRO TANG HW, 2007, ANAL CHEM, V79, P3646, DOI 10.1021/ac062362g NR 11 TC 0 PU OFFICE SPECTROSCOPY & SPECTRAL ANALYSIS PI BEIJING PA NO 76 COLLAGE SOUTH RD BEIJING, BEIJING 100081, PEOPLES R CHINA SN 1000-0593 J9 SPECTROSC SPECTR ANAL JI Spectrosc. Spectr. Anal. PD NOV PY 2010 VL 30 IS 11 BP 2981 EP 2984 DI 10.3964/j.issn.1000-0593(2010)11-2981-04 PG 4 SC Spectroscopy GA 694GW UT ISI:000285284000024 ER |
4Â¥2011-04-25 20:27:19














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