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liuyuncun

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Oysters and plankton were seeded with a GII.4 RNA run-off
transcript and RNA was extracted and purified to determine the
efficiency of each extraction method. For the silica-bead method,
2 μL of RNA transcript (2 × 106copies) was added and bound to the
size-fractionated silica after the oyster or plankton suspension was
treated with 900 l buffer L6. For the Qiagen kit, the flow-through
from the Qiashredder column was added to a new 1.5-ml tube and
seeded with 2 μL of the GII RNA (2 × 106copies).
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zuodg

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sltmac(金币+1): 谢谢交流~~ 2011-04-22 12:31:12
liuyuncun(金币+5, 翻译EPI+1): 2011-04-23 07:02:51
sltmac(金币+5): 2011-04-28 11:47:14
引用回帖:
Originally posted by liuyuncun at 2011-04-22 08:24:49:
Oysters and plankton were seeded with a GII.4 RNA run-off
transcript and RNA was extracted and purified to determine the
efficiency of each extraction method. For the silica-bead method,
2 μL o ...

用GII.4 RNA run-off transcript试剂盒收集牡蛎和浮游生物,提取、纯化RNA确定每种提取方法的效率。对于硅胶珠法,用900 l缓冲液L6处理牡蛎和浮游生物悬浮液,然后加入2 μLRNA transcript (2 × 106拷贝)使其结合到大小分粒级的硅胶上面。对于Qiagen试剂盒,将Qiashredde珠子置于新1.5-ml离心管中,用2 ul GII RNA(2 × 106拷贝)洗脱。
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