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liuyuncun金虫 (小有名气)
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[求助]
求助翻译一小段分子方面的文献
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Oysters and plankton were seeded with a GII.4 RNA run-off transcript and RNA was extracted and purified to determine the efficiency of each extraction method. For the silica-bead method, 2 μL of RNA transcript (2 × 106copies) was added and bound to the size-fractionated silica after the oyster or plankton suspension was treated with 900 l buffer L6. For the Qiagen kit, the flow-through from the Qiashredder column was added to a new 1.5-ml tube and seeded with 2 μL of the GII RNA (2 × 106copies). |
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zuodg
铁杆木虫 (正式写手)
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sltmac(金币+1): 谢谢交流~~ 2011-04-22 12:31:12
liuyuncun(金币+5, 翻译EPI+1): 2011-04-23 07:02:51
sltmac(金币+5): 2011-04-28 11:47:14
sltmac(金币+1): 谢谢交流~~ 2011-04-22 12:31:12
liuyuncun(金币+5, 翻译EPI+1): 2011-04-23 07:02:51
sltmac(金币+5): 2011-04-28 11:47:14
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用GII.4 RNA run-off transcript试剂盒收集牡蛎和浮游生物，提取、纯化RNA确定每种提取方法的效率。对于硅胶珠法，用900 l缓冲液L6处理牡蛎和浮游生物悬浮液，然后加入2 μLRNA transcript （2 × 106拷贝）使其结合到大小分粒级的硅胶上面。对于Qiagen试剂盒，将Qiashredde珠子置于新1.5-ml离心管中，用2 ul GII RNA（2 × 106拷贝）洗脱。 |
2楼2011-04-22 10:10:19