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Oysters and plankton were seeded with a GII.4 RNA run-off transcript and RNA was extracted and purified to determine the efficiency of each extraction method. For the silica-bead method, 2 ¦ÌL of RNA transcript (2 ¡Á 106copies) was added and bound to the size-fractionated silica after the oyster or plankton suspension was treated with 900 l buffer L6. For the Qiagen kit, the flow-through from the Qiashredder column was added to a new 1.5-ml tube and seeded with 2 ¦ÌL of the GII RNA (2 ¡Á 106copies). |
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Ìú¸Ëľ³æ (ÕýʽдÊÖ)
ÓÐΪÇàÄê
- ·ÒëEPI: 180
- Ó¦Öú: 5 (Ó×¶ùÔ°)
- ¹ó±ö: 0.015
- ½ð±Ò: 6765.2
- É¢½ð: 48
- ºì»¨: 18
- Ìû×Ó: 500
- ÔÚÏß: 285.5Сʱ
- ³æºÅ: 1055187
- ×¢²á: 2010-07-10
- ÐÔ±ð: GG
- רҵ: Íâ¹úÓïÑÔ
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sltmac(½ð±Ò+1): лл½»Á÷~~ 2011-04-22 12:31:12
liuyuncun(½ð±Ò+5, ·ÒëEPI+1): 2011-04-23 07:02:51
sltmac(½ð±Ò+5): 2011-04-28 11:47:14
sltmac(½ð±Ò+1): лл½»Á÷~~ 2011-04-22 12:31:12
liuyuncun(½ð±Ò+5, ·ÒëEPI+1): 2011-04-23 07:02:51
sltmac(½ð±Ò+5): 2011-04-28 11:47:14
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ÓÃGII.4 RNA run-off transcriptÊÔ¼ÁºÐÊÕ¼¯Äµòú͸¡ÓÎÉúÎÌáÈ¡¡¢´¿»¯RNAÈ·¶¨Ã¿ÖÖÌáÈ¡·½·¨µÄЧÂÊ¡£¶ÔÓڹ轺Öé·¨£¬ÓÃ900 l»º³åÒºL6´¦Àíĵòú͸¡ÓÎÉúÎïÐü¸¡Òº£¬È»ºó¼ÓÈë2 ¦ÌLRNA transcript £¨2 ¡Á 106¿½±´£©Ê¹Æä½áºÏµ½´óС·ÖÁ£¼¶µÄ¹è½ºÉÏÃæ¡£¶ÔÓÚQiagenÊÔ¼ÁºÐ£¬½«QiashreddeÖé×ÓÖÃÓÚÐÂ1.5-mlÀëÐĹÜÖУ¬ÓÃ2 ul GII RNA£¨2 ¡Á 106¿½±´£©Ï´ÍÑ¡£ |

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