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ydd1982

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[ÇóÖú] Ó¢ÎÄÎÄÏ×ÐèÒª·­Òëһϣ¬Ë­ÄܰïÏÂæ

Cultivable diversity of bacteria associated with
citrus was investigated as part of a larger study to
understand the roles of beneficial bacteria and utilize them
to increase the productive capacity and sustainability of
agro-ecosystems. Citrus roots from Huanglongbing (HLB)
diseased symptomatic and asymptomatic citrus were used
in this study. A total of 227 and 125 morphologically
distinct colonies were isolated and characterized from HLB
asymptomatic and symptomatic trees, respectively. We
observed that the frequency of bacterial isolates possessing
various plant beneficial properties was significantly higher
in the asymptomatic samples. A total of 39 bacterial isolates
showing a minimum of five beneficial traits related to
mineral nutrition [phosphate (P) solubilization, siderophore
production, nitrogen (N) fixation], development [indole acetic
acid (IAA) synthesis], health [production of antibiotic and
lytic enzymes (chitinase)], induction of systemic resistance
[salicylic acid (SA) production], stress relief [production of
1-amino-cyclopropane-1-carboxylate deaminase] and production
of quorum sensing [N-acyl homoserine lactones]
signals were characterized. A bioassay using ethidium
monoazide (EMA)-qPCR was developed to select bacteria
antagonistic to Candidatus Liberibacter asiaticus. Using
the modified EMA-qPCR assay, we found six bacterial
isolates showing maximum similarity to Paenibacillus

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shirongjiu

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ydd1982(½ð±Ò+15, ·­ÒëEPI+1): µÚÒ»¾ä»°ºÃÏñ»¹¿ÉÒÔ׼ȷһЩ 2011-04-18 23:42:49
sltmac(½ð±Ò+15): лл~~~ 2011-04-21 23:16:16
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ydd1982

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Originally posted by shirongjiu at 2011-04-18 09:55:04:
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