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Cultivable diversity of bacteria associated with citrus was investigated as part of a larger study to understand the roles of beneficial bacteria and utilize them to increase the productive capacity and sustainability of agro-ecosystems. Citrus roots from Huanglongbing (HLB) diseased symptomatic and asymptomatic citrus were used in this study. A total of 227 and 125 morphologically distinct colonies were isolated and characterized from HLB asymptomatic and symptomatic trees, respectively. We observed that the frequency of bacterial isolates possessing various plant beneficial properties was significantly higher in the asymptomatic samples. A total of 39 bacterial isolates showing a minimum of five beneficial traits related to mineral nutrition [phosphate (P) solubilization, siderophore production, nitrogen (N) fixation], development [indole acetic acid (IAA) synthesis], health [production of antibiotic and lytic enzymes (chitinase)], induction of systemic resistance [salicylic acid (SA) production], stress relief [production of 1-amino-cyclopropane-1-carboxylate deaminase] and production of quorum sensing [N-acyl homoserine lactones] signals were characterized. A bioassay using ethidium monoazide (EMA)-qPCR was developed to select bacteria antagonistic to Candidatus Liberibacter asiaticus. Using the modified EMA-qPCR assay, we found six bacterial isolates showing maximum similarity to Paenibacillus |
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shirongjiu
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ydd1982(金币+15, 翻译EPI+1): 第一句话好像还可以准确一些 2011-04-18 23:42:49
sltmac(金币+15): 谢谢~~~ 2011-04-21 23:16:16
ydd1982(金币+15, 翻译EPI+1): 第一句话好像还可以准确一些 2011-04-18 23:42:49
sltmac(金币+15): 谢谢~~~ 2011-04-21 23:16:16
| 为了解有益细菌并利用它们提高农业生态系统生产能力及可持续发展性,本文选取有黄龙病症状与无病症状的柑橘树根,研究了与柑橘树有关的可培养的细菌多样性。(研究结果显示:)在有病及无病树根上分别分离到227和125个有显著形态差异的菌落。在无病的柑橘树根上分离到对树木有益的细菌菌落的频率高于有病的处理。总计有39个细菌菌株具有至少五种对树木生长有益的特性,如矿物营养(磷溶解、含铁细胞形成、固氮)、健康(产抗生素及溶解酶(几丁质酶)、诱导协同抗性(产水杨酸)、抗压(产生1-氨基环丙烷-1-羧基脱氨酶)、产生群体感应信号分子(N-酰基高丝氨酸内酯)。此外,建立了EMA-qPCR方法用以筛选对柑橘溃疡病菌(Candidatus Liberibacter asiaticus)有抗性的细菌菌株。通过优化该方法,发现有6株细菌与类芽孢杆菌有最大相似性。 |
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