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ÓÚ¿(½ð±Ò+50, ·ÒëEPI+1): 2011-04-10 13:02:20
| PSos-MLPKf2 bait plasmid was constructed and transformed into competent yeast. The results of yeast growth showed that the bait hadn¡¯t toxicity on the yeast cell growth. This bait could be used for screening experiments in the CytoTrap yeast two-hybrid system for the activation of the test showed that the bait had no self-activation. The filter efficiency of pSos-MLPKf2 bait plasmid and library plasmids were transformed into yeast competent was 104, which meet the test requirements. A positive clone was obtained after repeating screening. The sequenced tested by BLAST showed that the inserted sequence is the homologous sequence of SHV3 and the homology was 77%. The research provided a new clues for more in-depth research of MLPKf2 function and molecular mechanism of SI. |
4Â¥2011-04-09 21:57:24
2Â¥2011-04-09 21:42:29














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