Per liter
Bacto nutrient broth (Difco) 8 g
10% (w/v) KCl 10 ml
1.2% (w/v) MgSO4·7H2O 10 ml
1 M NaOH ~1.5 ml (pH to 7.6)
Adjust volume to 1 liter with ddH20. pH to 7.6. Autoclave and allow to cool to 50°C. Just prior to use, add the following sterile solutions (and antibiotics if required):
1 M Ca(NO3)2 1 ml
0.01 M MnCl2 1 ml
1 mM FeSO4 1 ml
回复此楼
+2/90
