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Tomato fruit were surface-sterilized with 2% (v/v) sodium hypochlorite for 2min, washed with tap water and air-dried. Fruit were immersed in 100uM MeJA and 100uM nordihydroguaiaretic acid (NDGA, Aldrich, Chemical Company, Wilwaukee, WI, USA) solutions respectively in a 50 L stainless steel vacuum container and vacuum infiltrated under low pressure (−35 kPa) for 0.5 min. Thereafter, fruit were kept in solution under air pressure for 2min and then air-dried. Water-treated fruit were used as controls. All fruit were then stored at 25±1 ◦C, 85–90% RH. Fruit samples were taken from5 fruit before treatment (time 0) and at 3, 6, 12 h within the first day after treatment and 1, 3, 6, 9 and 12 d during later storage for enzyme assays and measurements of jasmonate content, ethylene production, ACC content and superoxide anion contents. Each treatment contained three replicates of 45 fruit and the entire experiment was performed twice. [ Last edited by 昔年残梦 on 2011-4-1 at 18:40 ] |
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昔年残梦(金币+12, 翻译EPI+1): 2011-04-01 21:39:04
昔年残梦(金币+3): 2011-04-01 21:46:16
昔年残梦(金币+3): 2011-04-01 21:46:16
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用2%（体积比）次氯酸钠对番茄水果进行表面灭菌2min, 用蒸馏水冲洗并风干。水果分别浸没在装有100uM MeJA 和100uM nordihydroguaiaretic 酸（NDGA, Aldrich, Chemical Company, Wilwaukee, WI, USA ）溶液的体积为50L的不锈钢真空容器（压力低于−35 kPa是才会发生渗透）中半分钟。 然后，水果在常压下在溶液中保持2min后风干。用水处理的水果作为对照试验。所有的水果储存在温度25±1 ◦C，85–90% RH的条件下。 水果样品被取出来5次，实验前(0h)、第一天的3、6、12h和处理后，在后面的酶试验储藏和茉莉酮酸酯、乙烯产量、ACC含量及过氧阴离子测试过程中，第1、3、6、9、12天进行取样。每个处理有45个水果的三个重复试验，整个试验进行两次。 仅供参考 |
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