24小时热门版块排行榜

返回列表

当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖

mao2mao

木虫 (小有名气)

应助: 1 (幼儿园)
金币: 6343.1
帖子: 129
在线: 37.1小时
虫号: 863818

[交流] 【请教】请问一下纳米二氧化硅如何酸化呢？

最近想做一些纳米二氧化硅的改性，请问用硅烷偶联剂改性后，其表面接的氨基如何进一步变为羧基呢？希望有人提供些经验或者文献。

[ Last edited by zhangwj on 2011-3-27 at 17:06 ]

回复此楼

» 收录本帖的淘帖专辑推荐

硅球方面的帖子

» 猜你喜欢

请问对标matlab的开源软件octave的网站https://octave.org为什么打不开？已经有1人回复
求助两种BiOBr晶体的CIF文件（卡片号为JCPDS 09-0393与JCPDS 01-1004 ）已经有0人回复
无机化学论文润色/翻译怎么收费? 已经有183人回复
哈尔滨工程大学材化学院国家级青年人才-26年硕士招生已经有0人回复

» 本主题相关商家推荐: (我也要在这里推广)

» 本主题相关价值贴推荐，对您同样有帮助:

没投射电镜的情况下如何知道是否连接上二氧化硅已经有10人回复
大家说一下表征四氧化三铁和包裹二氧化硅的四氧化三铁的TEM型号，非常非常重要已经有10人回复
请问下氢氟酸除去二氧化硅的问题已经有11人回复
合成介孔二氧化硅我采用了CTAC这种模板，怎么除去呢已经有14人回复
求助0.06%纳米二氧化硅水溶液的配置问题已经有10人回复
【讨论】这个“纳米二氧化硅”也太搞了，如何造假 Nano SiO2 已经有27人回复
【讨论】关于制备金纳米离子包裹二氧化硅壳核材料已经有48人回复
【求助】请教可以合成出纳米二氧化硅晶体吗？已经有3人回复
【求助】纳米二氧化硅的改性及分散问题已经有49人回复

» 抢金币啦！回帖就可以得到:

查看全部散金贴

1楼 2011-03-27 17:01:34

已阅回复此楼关注TA 给TA发消息送TA红花 TA的回帖

SDK840715

新虫

应助: 0 (幼儿园)
金币: 6
在线: 26.9小时
虫号: 494251

★
mao2mao(金币+1):谢谢参与
mao2mao(金币+3): 谢谢赐教哦~~~ 2011-10-09 13:42:47

楼主看文献不仔细啊。请看这里。

Park, H. S., C. W. Kim, et al. (2010). "A mesoporous silica nanoparticle with charge-convertible pore walls for efficient intracellular protein delivery." Nanotechnology 21(22): 225101.
We report a smart mesoporous silica nanoparticle (MSN) with a pore surface designed to undergo charge conversion in intracellular endosomal condition. The surface of mesopores in the silica nanoparticles was engineered to have pH-hydrolyzable citraconic amide. Solid-state nuclear magnetic resonance (NMR), Fourier-transform infrared (FT-IR) spectroscopy, and Brunauer-Emmett-Teller (BET) analyses confirmed the successful modification of the pore surfaces. MSNs (MSN-Cit) with citraconic amide functionality on the pore surfaces exhibited a negative zeta potential (-10 mV) at pH 7.4 because of the presence of carboxylate end groups. At cellular endosomal pH (approximately 5.0), MSN-Cit have a positive zeta potential (16 mV) indicating the dramatic charge conversion from negative to positive by hydrolysis of surface citraconic amide. Cytochrome c (Cyt c) of positive charges could be incorporated into the pores of MSN-Cit by electrostatic interactions. The release of Cyt c can be controlled by adjusting the pH of the release media. At pH 7.4, the Cyt c release was retarded, whereas, at pH 5.0, MSN-Cit facilitated the release of Cyt c. The released Cyt c maintained the enzymatic activity of native Cyt c. Hemolytic activity of MSN-Cit over red blood cells (RBCs) was more pronounced at pH 5.0 than at pH 7.0, indicating the capability of intracellular endosomal escape of MSN carriers. Confocal laser scanning microscopy (CLSM) studies showed that MSN-Cit effectively released Cyt c in endosomal compartments after uptake by cancer cells. The MSN developed in this work may serve as efficient intracellular carriers of many cell-impermeable therapeutic proteins.