Originally posted by kyssishino at 2011-03-29 12:33:54:
用羧酸酐和氨基反应形成酰胺，链的终端应该就是带羧基的了

你看这样行不

我最近也在做氨基硅烷偶联剂二氧化硅表面改性，需要接一个羧基形成酰胺，不过我感觉我的不太好接，有什么可以继续交流

我也见过用酸酐与氨基发生反应，变酰胺，然后外部就有羧基，可是没有文献中描述的那样好做。

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11楼2011-03-29 14:15:14

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phu_grassman

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mao2mao(金币+2): 非常感谢~~ 2011-03-29 15:42:57

CTES carboxyethylsilanetriol

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12楼2011-03-29 14:52:38

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mao2mao(金币+1):谢谢参与
mao2mao(金币+3): 谢谢赐教哦~~~ 2011-10-09 13:42:47

楼主看文献不仔细啊。请看这里。

Park, H. S., C. W. Kim, et al. (2010). "A mesoporous silica nanoparticle with charge-convertible pore walls for efficient intracellular protein delivery." Nanotechnology 21(22): 225101.
We report a smart mesoporous silica nanoparticle (MSN) with a pore surface designed to undergo charge conversion in intracellular endosomal condition. The surface of mesopores in the silica nanoparticles was engineered to have pH-hydrolyzable citraconic amide. Solid-state nuclear magnetic resonance (NMR), Fourier-transform infrared (FT-IR) spectroscopy, and Brunauer-Emmett-Teller (BET) analyses confirmed the successful modification of the pore surfaces. MSNs (MSN-Cit) with citraconic amide functionality on the pore surfaces exhibited a negative zeta potential (-10 mV) at pH 7.4 because of the presence of carboxylate end groups. At cellular endosomal pH (approximately 5.0), MSN-Cit have a positive zeta potential (16 mV) indicating the dramatic charge conversion from negative to positive by hydrolysis of surface citraconic amide. Cytochrome c (Cyt c) of positive charges could be incorporated into the pores of MSN-Cit by electrostatic interactions. The release of Cyt c can be controlled by adjusting the pH of the release media. At pH 7.4, the Cyt c release was retarded, whereas, at pH 5.0, MSN-Cit facilitated the release of Cyt c. The released Cyt c maintained the enzymatic activity of native Cyt c. Hemolytic activity of MSN-Cit over red blood cells (RBCs) was more pronounced at pH 5.0 than at pH 7.0, indicating the capability of intracellular endosomal escape of MSN carriers. Confocal laser scanning microscopy (CLSM) studies showed that MSN-Cit effectively released Cyt c in endosomal compartments after uptake by cancer cells. The MSN developed in this work may serve as efficient intracellular carriers of many cell-impermeable therapeutic proteins.

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13楼2011-08-03 10:42:04

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