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小木虫论坛-学术科研互动平台» 学术交流区» 论文翻译 » 论文翻译求助完结子版 » difficult proteins ？
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explore

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[交流] difficult proteins ？

文献中出现 difficult proteins 是什么意思？怎么翻译？谢谢！
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1楼 2011-03-10 15:27:11
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zerohead

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difficult proteins

Difficult proteins are those which the biology suggests are present in the sample, but remain undetected in the analysis.

困难蛋白：是指那些在生物学理论上存在的蛋白，但仍然未能在研究分析中合成或发现。
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3楼2011-03-10 15:54:46
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qjluo

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explore(金币+5, 翻译EPI+1): 谢谢 2011-03-11 09:34:00
看你具体语境吧。

在Pubmed终查到一篇篇名叫difficult proteins 的综述

摘要内容如下：
The degree of protein diversity and dynamic range within organisms means that even the simplest proteome cannot be captured by any single extraction and separation step. New techniques have focused on major protein classes often under-represented in proteome analysis; low abundance, membrane, and alkaline proteins. The last decade has seen considerable technology development in fractionation tools aimed at complexity reduction in many forms. The key outcome of complexity reduction is that each fraction, or sub-proteome, can be studied in more detail, and proteins which would have remained undetected in a total extract are present in sufficient quantities. However, the tools available are fractionations, not amplifications, and like all mining for rare and difficult items, a large amount of starting material is often required. The key shortcomings of many proteome analysis techniques are now well documented. With this knowledge, the best modern proteomics 'platform' involves combining multiple protein extractions, gel and chromatographic separations, and multiple MS analysis methods.


单纯从这个文章的内容来看，difficult proteins 意思就是“难对付/处理的蛋白质”
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2楼2011-03-10 15:42:54
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