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ringzhu(金币+50, 翻译EPI+1): ——！ 2011-03-22 17:30:27
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several immunologic assays have been proposed for Ep including a hemagglutination inhibition assay and a radioimmunoassay . 已经提出了包括血凝抑制试验和放射免疫法在内的几个红细胞生成素的免疫检测方法。The hemagglutination inhibition system has been commercially marked, but has many serious problems , which appear to be related to the specificity of the antiserum. 血凝抑制系统已被商业注册，但存在许多严重的问题，这似乎是与抗血清的特异性有关。Two different laboratories have developed radioimmunoassays ,which utilize highly purified Ep preparations (8000-12,000units/mg)labeled with I. 两各不同的实验室已开发出放射免疫测定方法，它利用以碘标记的高纯度红细胞生成素制剂（8000-12,000单位/毫克）The assays appear to be sufficiently sensitive to quantitate normal serum Ep levels , but the range of normal found in the two laboratories varied considerably. 这种检测方法对定量正常血清水平红细胞生成素似乎是足够敏感的，但这一正常范围在两个实验室的发现相去甚远。Both groups observed that patients with renal failure had very high serum Ep levels by radioimmunoassay even though several of the same patients had no detectable activity by the polycythemic mouse assay. 尽管其中有几个病人用多血小鼠方法测不到有活性的红细胞生成素，但是两组均观察到用放射免疫法可以测到肾功能衰竭患者有非常高的红细胞生成素水平。In addition , it was shown that asialo-Ep was immunoreactive, but inactive in vivo .此外，促红细胞生成素具有免疫活性但无体内活性。These studies suggested that serum from patients with renal failure might contain an immunoreactive but biologically inactive Ep. 这些研究表明，肾功能衰竭患者血清可能包含具有免疫反应性但生物无效的红细胞生成素。This is a very interesting bypothesis and , if confirmed , may increase our understanding of the anemia of renal disease . 这是一个非常有趣的假设，如果得到证实，可能会增加我们对肾性贫血病的认识。Much more correlation of the bioassay and radioimmunoassay in diverse clinical situations is needed before the latter can be used for routine assay of the hormone.更多的生物测定和放射免疫分析之间的相关性需要在不同临床情况下加以确定，只有如此，放射免疫方法才可用于激素的常规检测。 |
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