| 查看: 1167 | 回复: 5 | |||
| 本帖产生 1 个 翻译EPI ，点击这里进行查看 | |||
[交流]
生医药专业翻译
|
|||
|
the standard unit of measure for Ep is based on the erythropoietic effect of cobalt. Injection of cobalt into starved rats produces a dose-related increase in erythropoiesis identical to that produced by crude Ep. Inorder to provide for a consistent, easily obtainable standard, one unit of Ep was set equal to the response of 5 mmoles of cobalt in the fasted-rat assay.When sheep Ep extracts became available , one batch was set aside as Standard A.one unit of Standard A was 0.05mg and it produced a response equivalent to one cobalt unit . As this material was used up it was replaced by standard B,a lyophilized crude preparation of human urine Ep.The international unit for Ep is currently 1.48mg of Standard B, which produces a response similiar to that observed with one cobalt unit or one unit of Standard A. Tissue culture assays have been developed that are very sensitive to Ep and give excellent dose response curves . These systems utilize either rat marrow or fetal liver cells and are based on incorporation of Fe into heme .Amounts of Ep as small as 0.001 to 0.005 units can be measured .Recently , a plasma clot culture technique using mouse or human marrow cells has been described in which erthroid colonies form in vivtro . This system also responds to Ep in a dose-related fashion and could be used to assay the hormone .These methods , however , may detect asialo-Ep, which is inactive in vivo , and may be sensitive to other factors in serum and urine , making it difficult to determine the amount of Ep in crude preparations . They have been of value in following the Ep concentration in diverse fractions produced by purification procedures and in doing stability studies,but ultimately the polycythemic mouse assay must be used for definitive measurement of the hormone . several immunologic assays have been proposed for Ep including a hemagglutination inhibition assay and a radioimmunoassay . The hemagglutination inhibition system has been commercially marked, but has many serious problems , which appear to be related to the specificity of the antiserum. Two different laboratories have developed radioimmunoassays ,which utilize highly purified Ep preparations (8000-12,000units/mg)labeled with I.The assays appear to be sufficiently sensitive to quantitate normal serum Ep levels , but the range of normal found in the two laboratories varied considerably. Both groups observed that patients with renal failure had very high serum Ep levels by radioimmunoassay even though several of the same patients had no detectable activity by the polycythemic mouse assay. In addition , it was shown that asialo-Ep was immunoreactive, but inactive in vivo .These studies suggested that serum from patients with renal failure might contain an immunoreactive but biologically inactive Ep. This is a very interesting bypothesis and , if confirmed , may increase our understanding of the anemia of renal disease .Much more correlation of the bioassay and radioimmunoassay in diverse clinical situations is needed before the latter can be used for routine assay of the hormone. |
回复此楼» 猜你喜欢
求调剂推荐
已经有7人回复
-
289 分105500药学专硕求调剂(找B区学校)
已经有4人回复
-
0854求调剂
已经有13人回复
-
初试324 中药学 一志愿天中医 求调剂
已经有3人回复
-
药学求调剂
已经有14人回复
-
327求调剂
已经有27人回复
-
急需调剂
已经有5人回复
-
300求调剂
已经有7人回复
-
271求调剂
已经有33人回复
-
273求调剂
已经有8人回复
» 抢金币啦！回帖就可以得到:
-
科研需求-硫化镍钴精矿样品
+1/399
-
坐标山东，诚征女友
+1/357
-
教程+代算 GROMACS模拟蛋白质-小分子、药物自组装、高分子弛豫、分子转运等
+1/89
-
物理学调剂
+1/89
-
武汉纺织大学材料学院士兵计划调剂信息
+1/86
-
五邑大学 化学工程与技术调剂 4月10号九点截止！！！！！
+1/41
-
江西科技师范大学086生物与医药最后7个，今晚线上面试
+1/41
-
北华大学 08代码 调剂虚位以待
+1/37
-
中新绿色科技研究院（山东济南）招收机械设计方向实习生
+1/36
-
浙江理工大学蒋仲庆教授课题组招收物理或者材料背景研究生（调剂1名）
+1/16
-
山东大学集成电路学院招博士了
+1/10
-
广东石油化工学院生物与医药专硕仍有部分调剂名额，欢迎农，工，理和医学的同学调剂。
+1/9
-
省重点实验室招收07专业调剂生，海洋生物专业名额充足
+1/9
-
西安工业大学材料与化工专业先进电子材料与器件课题组专业博士招生
+1/8
-
山东大学集成电路学院招博士了
+1/7
-
湖南理工大学化学化工学院的化学和化学工程与技术专业调剂 最后一次开放系统
+1/7
-
淡水养殖英语刊物
+1/3
-
中国海洋大学工程学院科研博士后招聘启事（数学/能源化工方向）
+1/3
-
上海市过程安全与智能控制重点实验室招收安全工程研究生调剂-上岸既是高质量就业！
+1/2
-
安徽工程大学化学与环境工程学院2026级硕士研究生还有部分调剂名额-资源与环境专硕
+1/1
★ ★
ringzhu(金币-2): 这一段是机器翻译的吧.。。。 2011-03-22 17:29:45
ringzhu(金币-2): 这一段是机器翻译的吧.。。。 2011-03-22 17:29:45
| the standard unit of measure for Ep is based on the erythropoietic effect of cobalt. Ep的标准定位的测量是基于钴的促红细胞生成效应.Injection of cobalt into starved rats produces a dose-related increase in erythropoiesis identical to that produced by crude Ep.给饥饿大鼠注射钴产生与原Ep相同的剂量相关性红细胞生成的增加. Inorder to provide for a consistent, easily obtainable standard, one unit of Ep was set equal to the response of 5 mmoles of cobalt in the fasted-rat assay.为了提供一致、易于获得的标准，一个单位的Ep设定为与5毫摩尔钴在禁食大鼠测定中的响应相当。When sheep Ep extracts became available , one batch was set aside as Standard A. 当绵羊Ep提取物可以获得时，一批被搁置一边作为标准A。one unit of Standard A ，was 0.05mg and it produced a response equivalent to one cobalt unit . 一个单位的标准A为0.05mg，它产生一个响应相当于一个钴单位。As this material was used up it was replaced by standard B,a lyophilized crude preparation of human urine Ep.当这种材料用完后就用标准B-一种人尿来源的冻干粗制品替代。The international unit for Ep is currently 1.48mg of Standard B, which produces a response similiar to that observed with one cobalt unit or one unit of Standard A.当前Ep的国际单位是1.48mg标准B，其产生的反应与一个钴单位或者一个单位的A标准相似。 |
3楼2011-03-06 23:03:57
4楼2011-03-06 23:05:32
| Tissue culture assays have been developed that are very sensitive to Ep and give excellent dose response curves . 已经建立了组织培养测定方法，对红细胞生成素十分灵敏，可以得到优良的剂量反应曲线。These systems utilize either rat marrow or fetal liver cells and are based on incorporation of Fe into heme . 这些系统利用大鼠骨髓或胎肝细胞，并以铁纳入血红素为基础。 Amounts of Ep as small as 0.001 to 0.005 units can be measured . 小到0.001到0.005单位的红细胞生成素也可以测得。Recently , a plasma clot culture technique using mouse or human marrow cells has been described in which erthroid colonies form in vivtro . 最近，已有报道使用鼠或人类骨髓细胞血浆凝块培养技术在体外形成幼红细胞克隆。This system also responds to Ep in a dose-related fashion and could be used to assay the hormone . 该系统也以剂量相关的方式对红细胞生成素产生响应，可以用来检测激素。These methods , however , may detect asialo-Ep, which is inactive in vivo , and may be sensitive to other factors in serum and urine , making it difficult to determine the amount of Ep in crude preparations . 但是这些方法，可能会检测到促红细胞生成素，促红细胞生成素在体内无活性，可能对血清和尿液中其它因子敏感，因此很难确定粗制剂中的红细胞生成素的含量。They have been of value in following the Ep concentration in diverse fractions produced by purification procedures and in doing stability studies, but ultimately the polycythemic mouse assay must be used for definitive measurement of the hormone .在对纯化过程和稳定性研究中跟踪红细胞生成素的浓度，这些方法具有重要价值，但最终准确测量激素还必须通过多血小鼠方法检测。 |
5楼2011-03-07 08:21:46
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
ringzhu(金币+50, 翻译EPI+1): ——！ 2011-03-22 17:30:27
ringzhu(金币+50, 翻译EPI+1): ——！ 2011-03-22 17:30:27
| several immunologic assays have been proposed for Ep including a hemagglutination inhibition assay and a radioimmunoassay . 已经提出了包括血凝抑制试验和放射免疫法在内的几个红细胞生成素的免疫检测方法。The hemagglutination inhibition system has been commercially marked, but has many serious problems , which appear to be related to the specificity of the antiserum. 血凝抑制系统已被商业注册，但存在许多严重的问题，这似乎是与抗血清的特异性有关。Two different laboratories have developed radioimmunoassays ,which utilize highly purified Ep preparations (8000-12,000units/mg)labeled with I. 两各不同的实验室已开发出放射免疫测定方法，它利用以碘标记的高纯度红细胞生成素制剂（8000-12,000单位/毫克）The assays appear to be sufficiently sensitive to quantitate normal serum Ep levels , but the range of normal found in the two laboratories varied considerably. 这种检测方法对定量正常血清水平红细胞生成素似乎是足够敏感的，但这一正常范围在两个实验室的发现相去甚远。Both groups observed that patients with renal failure had very high serum Ep levels by radioimmunoassay even though several of the same patients had no detectable activity by the polycythemic mouse assay. 尽管其中有几个病人用多血小鼠方法测不到有活性的红细胞生成素，但是两组均观察到用放射免疫法可以测到肾功能衰竭患者有非常高的红细胞生成素水平。In addition , it was shown that asialo-Ep was immunoreactive, but inactive in vivo .此外，促红细胞生成素具有免疫活性但无体内活性。These studies suggested that serum from patients with renal failure might contain an immunoreactive but biologically inactive Ep. 这些研究表明，肾功能衰竭患者血清可能包含具有免疫反应性但生物无效的红细胞生成素。This is a very interesting bypothesis and , if confirmed , may increase our understanding of the anemia of renal disease . 这是一个非常有趣的假设，如果得到证实，可能会增加我们对肾性贫血病的认识。Much more correlation of the bioassay and radioimmunoassay in diverse clinical situations is needed before the latter can be used for routine assay of the hormone.更多的生物测定和放射免疫分析之间的相关性需要在不同临床情况下加以确定，只有如此，放射免疫方法才可用于激素的常规检测。 |
6楼2011-03-07 08:53:08
简单回复
一直寻觅2楼
2011-03-06 00:38
回复
