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The current assay(13) utilizes either hypertransfused of exhypoxic mice . The above-normal red cell concentration produces an almost complete cessation of erythropoiesis .Ep or a test substance is then administered and this is followed in one to three days by parenteral  Fe. Three days later animals are bled and the amount of Fe in red cells determined. This system can detect small amounts of the hormone and has a 40-fold range of response , but it cannot measure the amount of hormone in normal serum. For normal individuals and patients with reduced or only slightly increased amounts of Ep, concentrated urine specimens must be assayed to measure the level of hormone . This assay also suffers from a lack of precision ,is time consuming , and requires the use of large numbers of mice per group . In addition , androgens ,cobalt, certainvasoactive amines (such as histamine, tryptomine, and serotomin), prostagladins , cyclic nucleotides ,and agents that cause hemolysis may increase erythropoiesis and produce false-positive results .Nevertheless , with proper controls this is overall the most reliable and widely used assay available.

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