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The current assay(13) utilizes either hypertransfused of exhypoxic mice . The above-normal red cell concentration produces an almost complete cessation of erythropoiesis .Ep or a test substance is then administered and this is followed in one to three days by parenteral  Fe. Three days later animals are bled and the amount of Fe in red cells determined. This system can detect small amounts of the hormone and has a 40-fold range of response , but it cannot measure the amount of hormone in normal serum. For normal individuals and patients with reduced or only slightly increased amounts of Ep, concentrated urine specimens must be assayed to measure the level of hormone . This assay also suffers from a lack of precision ,is time consuming , and requires the use of large numbers of mice per group . In addition , androgens ,cobalt, certainvasoactive amines (such as histamine, tryptomine, and serotomin), prostagladins , cyclic nucleotides ,and agents that cause hemolysis may increase erythropoiesis and produce false-positive results .Nevertheless , with proper controls this is overall the most reliable and widely used assay available.

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wjmsicuan(金币+10, 翻译EPI+1): 翻译的有些生硬,希望多看,多练习! 2011-03-05 10:50:16
目前的检测方法(13)采用任何hypertransfused的exhypoxic小鼠。上述正常红细胞浓度使得红血球生成几乎完全停止。EP或测试物质在给予一到三天肠外铁之后给予。三天后,取动物血确定铁红细胞数量。该系统可以检测到少量的激素,具有40倍范围内的响应,但它不能测量正常血清中的激素量。对于一般的EP量减少或只有轻微增加的正常个体和病人,尿液标本必须浓缩后检测以测到激素。此法准确性有限,非常耗时,而且要求每个组小鼠有较大数量。此外,雄激素,钴,某些血管活性胺(如组胺,tryptomine和5-羟色胺),前列腺素,环核苷酸和某些可能导致溶血的试剂,都会增加红细胞生成和造成假阳性结果。尽管如此,在适当的对照下,这种方法从整体来说是目前可用最可靠和广泛使用的检测方法。
2楼2011-03-04 08:16:15
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