|
|
|
The initial work on purification of Ep was performed with plasma from anemic sheep,since relatively large volumes could be obtained and the presence of the hormone in urine was uncertain .Such plasma had a potency of 0.007 units/mg of protein ,and after an extensive sequence of purification procedures, Ep with a protency of 8250 units/mg was isolated(5).however,the final yield was only 0.4%.While the purification of Ep from plasma was proceeding ,it became evident that urine of anemic patients provided a source of hormone with much less contaminatiing protein. Their recovery was 18.8%,or more than 50 times greater than that with plasma Ep.Miyake, Kung&Goldwasser(7) have since applied the methodology they developed for purifying sheep palsma Ep to human urinary preparations. By a combination of treatment with DEAE-cellulose ,phenol-p-aminosalicylate , ethanol precipitation, DEAE-agarose, sulfopropyl-Sephadex,gel permeation, and hydroxylapatite chromatography, they obtained Ep at a potency of 70,400 units/mg of protein with a 21%yield. |
|
回复此楼
