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The CHO cells supernatant was added in the end and consisted of 25% (w/w) of the final ATPS weight. Partition assays were set up in 15mL graduated centrifuge tubes. All system components were then thoroughly mixed in a vortex (Ika, Staufen, Germany), incubated at 25 ◦C overnight and centrifuged at 3000rpm for 10min (Eppendorf, Hamburg, Germany). Phase volumes were determined and samples from the top and bottom were taken for phase composition analysis. All samples were analysed against blank systems containing the same phase composition but without the supernatant feed stock. |
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