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da_da

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The CHO cells supernatant was added in
the end and consisted of 25% (w/w) of the final ATPS weight. Partition
assays were set up in 15mL graduated centrifuge tubes. All
system components were then thoroughly mixed in a vortex (Ika,
Staufen, Germany), incubated at 25 ◦C overnight and centrifuged at
3000rpm for 10min (Eppendorf, Hamburg, Germany). Phase volumes were
determined and samples from the top and bottom were
taken for phase composition analysis. All samples were analysed
against blank systems containing the same phase composition but
without the supernatant feed stock.

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1楼 2011-02-28 21:40:36

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da_da(金币+10, 翻译EPI+1): 2011-03-01 10:22:55

最后加入占最终ATPS体系25％的（W / w）CHO细胞培养上清液。分配实验在15毫升刻度离心管中进行。随后所有系统组分彻底涡旋混合（Ika, Staufen,德国），25◦C过夜培养后，3000转离心10分钟（Eppendorf公司，德国汉堡）。确定相体积，并从顶相和底相取样进行分析，确定相组成。对所有样本分析均以含有相同的系统相组成、但没有加入CHO细胞培养上清液的空白进行对照。

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