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1.Within the concentration range investigated，TFA enhances peak shape，resolution，and retention of peptides when chromatographed by reversed-phase. 2.Formic acid can provide a reasonable substitute for TFA, yet permit sensitive MS detection. 3.Column inertness (lack of silanol activity) is a key criteria for optimal chromatographic performance under low or no TFA conditions. 4.Basic peptide probes reveal Discovery BIO Wide Pore to be a superior silica一based chromatographic matrix for operation under preferred conditions for MS一detection. 5.Evaluation with a typical complex peptide sample reveals Discovery BIO Wide Pore columns to compare most favorably with the competition. 6.The Discovery BIO Wide Pore reversed一phase family provides an ideal product for polypeptide chromatography under low or no TFA conditions. |
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