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aswman08(金币+5, 翻译EPI+1): 2011-01-26 08:44:27
According to the State Food and Drug Administration"Fingerprints of TCM injections Technical Guide","chromatographic fingerprint of traditional Chinese medicine injections Experimental Technology Guide " and the relevant literature, we determined the best chromatographic conditions of the HPLC fingerprint of American ginseng leaves by fingerprint analysis experiment .
Column: octadecyl silane bonded silica as a filler;
Mobile phase: A: acetonitrile, B: water (0.05% phosphoric acid, 2% tetrahydrofuran).
Column temperature:40 ℃
Injection volume: 20μL,
Flow rate :1.0ml/min
Detection wavelength: 203nm; 0 ~ 18min A: 20%; 18 ~ 58min, A: 20 ~ 40% (linear gradient); 58 ~ 73min, A: 40 ~ 100% (linear gradient); 73 ~ 80min, 100%. |
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