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羡东堡(金币+5, 翻译EPI+1):可能是我没写清楚，我要的是翻译。 2010-12-27 14:33:39
ringzhu(金币+2, 翻译EPI-1):元旦快乐~~· 2011-01-01 13:41:33
没啥问题，改了一个地方
The cells are washed in cold sterile distilled water and dehydrated by precipitating with three volumes of acetone at –20°C, and then they are allowed to stand at –20°C for 24–48 hours. After repeated washing in cold acetone, followed by a final rinse in diethyl ether, the cells are dried over calcium chloride and held at 4°C. The dried cells are subjected to a viability check. They are resuspended in sterile 2.5% sodium chloride to a final concentration of 5% (w/v) and agitated for 3 days at 4°C. |
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