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dabizi_911(½ð±Ò+1):лл²ÎÓë
lstt09nk(½ð±Ò+1):¹ÄÀø½»Á÷ 2010-11-16 12:51:54
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Originally posted by dabizi_911 at 2010-11-11 16:45:40:
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2Â¥2010-11-16 09:46:05
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silicare

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3Â¥2010-11-16 11:58:59
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caitikuan

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dabizi_911(½ð±Ò+1):лл²ÎÓë
lstt09nk(½ð±Ò+3):ÐÁ¿à 2010-11-18 10:54:54
dabizi_911(½ð±Ò+10): 2010-11-19 00:32:38
Brain Heart Infusion Broth is used for the cultivation fastidious microorganisms, including streptococci, pneumococci and meningococci from a variety of clinical and non-clinical materials.

Description:
Brain Heart Infusion (BHI) Broth is a modification of the media described by Rosenow and Hayden in which infusion from calf brains has replaced the brain tissue and disodium phosphate has replaced the calcium carbonate buffer. The addition of 0.1% agar can be used to lower oxygen tension to enhance the growth of anaerobic organisms.

Storage:
Store the sealed bottle containing the dehydrated medium at 2 to 30.0¡ãC. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.


Directions: 1. Dissolve 37grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.

2. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi). Storage:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4¡ãC and used within a short period of time.

Application: Cultivation of fastidious aerobic and anaerobic bacteria.

Appearance: Beige, homogeneous, free flowing powder.

pH (25¡ãC): 7.4¡À0.2

Microbiological Tests: Microorganisms

Components shown as g/liter
  
Brain Heart Infusion (from solids) 17.5
Pancreatic Digest of Gelatin 10.0
Sodium Chloride 5.0
Dextrose 2.0
Disodium Phosphate 2.5
  
Total: 37g/L

http://www.usbio.net/technicalSheet.php?item=B2701-07

[ Last edited by caitikuan on 2010-11-18 at 10:45 ]
6Â¥2010-11-18 10:44:16
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2010-11-18 09:46   »Ø¸´  
dabizi_911(½ð±Ò+1):лл²ÎÓë
lstt09nk:½»Á÷ÌùÇëÎð´¿±í 2010-11-18 10:54:08
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